Technical/dosimetric study (experimental study)

Acute and chronic effects of exposure to a 1-mT magnetic field on the cytoskeleton, stress proteins, and proliferation of astroglial cells in culture med./bio.

By: Bodega G, Forcada I, Suarez I, Fernandez B

Published in: Environ Res 2005; 98 (3): 355-362

Aim of study (acc. to author)

To examine the effects of exposure to magnetic fields on astroglial cells in culture.

Background/further details

1) The level of stress proteins HSP25, HSP60, and HSP70 and the cytoskeletal proteins actin and GFAP were determined after exposure to a 1-mT sinusoidal or static magnetic field or combined magnetic field for 1 hour. 2) The effect on the proteins after exposure to combined static/sinusoidal magnetic field for 1, 2, and 4 hours was examined. 3) The effect of a chronic (11-day) exposure to a combined magnetic field on the proliferation of cultured astroglial cells was investigated.

Endpoint

molecular biosynthesis: level of stress proteins and cytoskeletal proteins
cell viability/cell division/proliferation: proliferation

Exposure

Exposure	Parameters
Exposure 1: DC/static Exposure duration: continuous for 1 h Exposure to DC field	magnetic flux density: 1 mT (DC)
Exposure 2: 50 Hz Exposure duration: continuous for 1 h Combination of AC and DC field	magnetic flux density: 1 mT effective value (AC for experiment 1) magnetic flux density: 1 mT (DC for experiment 2) magnetic flux density: 1 mT effective value (AC + 1 mT DC for experiment 3)
Exposure 3: 50 Hz Exposure duration: continuous for 1 h, 2 h and 4 h Combination of AC and DC field	magnetic flux density: 1 mT effective value (AC) magnetic flux density: 1 mT (DC)
Exposure 4: 50 Hz Exposure duration: continuous for 11 days Chronic exposure	magnetic flux density: 1 mT effective value (AC) magnetic flux density: 1 mT (DC)

Exposure 1

Main characteristics

Frequency	DC/static
Type	magnetic field
Exposure duration	continuous for 1 h
Additional info	Exposure to DC field

Exposure setup

Exposure source	coil(s)
Setup	pair of coils inside an incubator
Sham exposure	A sham exposure was conducted.

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	1 mT	-	measured	-	DC

Exposure 2

Main characteristics

Frequency	50 Hz
Type	magnetic field
Waveform	sinusoidal
Exposure duration	continuous for 1 h
Additional info	Combination of AC and DC field
Additional info	+ DC of the same magnitude

Exposure setup

Exposure source	same setup as exposure 1
Sham exposure	A sham exposure was conducted.

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	1 mT	effective value	measured	-	AC for experiment 1
magnetic flux density	1 mT	-	measured	-	DC for experiment 2
magnetic flux density	1 mT	effective value	measured	-	AC + 1 mT DC for experiment 3

Exposure 3

Main characteristics

Frequency	50 Hz
Type	magnetic field
Waveform	sinusoidal
Exposure duration	continuous for 1 h, 2 h and 4 h
Additional info	Combination of AC and DC field
Additional info	+ DC of the same magnitude

Exposure setup

Exposure source	same setup as exposure 1
Sham exposure	A sham exposure was conducted.

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	1 mT	effective value	measured	-	AC
magnetic flux density	1 mT	-	measured	-	DC

Exposure 4

Main characteristics

Frequency	50 Hz
Type	magnetic field
Waveform	sinusoidal
Exposure duration	continuous for 11 days
Additional info	Chronic exposure
Additional info	+ DC of the same magnitude

Exposure setup

Exposure source	same setup as exposure 1
Setup	field perpendicular to the flask base
Sham exposure	A sham exposure was conducted.

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	1 mT	effective value	measured	-	AC
magnetic flux density	1 mT	-	measured	-	DC

Exposed system:

intact cell/cell culture
astroglial cells of P0-P1 Wistar rats

Methods Endpoint/measurement parameters/methodology

molecular biosynthesis: level of stress proteins HSP25, HSP60, and HSP70 and cytoskeletal proteins actin and GFAP (immunoblotting, western blotting)
cell viability/cell division/proliferation: proliferation (cell density (optical microscopy))

Investigated system:

isolated bio./chem. substance
intact cell/cell culture

Time of investigation:

after exposure

Main outcome of study (acc. to author)

The results suggest that exposure to sinusoidal magnetic field, static magnetic field, or combined magnetic field (1 mT) has no significant effects on the level of cytoskeletal and stress proteins or on proliferation of astroglial cells in culture.

Study character:

technical/dosimetric study
experimental study
full/main study

Study funded by

FIS (Fondo de Investigaciones Sanitarias, Health Research Fund) (Instituto de Salud Carlos III., Ministerio de Sanidad y Consumo) (Carlos III Health Institute, Ministry of Health and Consumption), Spain
Instituto de Salud Carlos III., Ministerio de Sanidad y Consumo (Carlos III Health Institute, Ministry of Health and Consumption), Spain

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Su L et al. (2017): The effects of 50 Hz magnetic field exposure on DNA damage and cellular functions in various neurogenic cells
Wu X et al. (2014): Weak Power Frequency Magnetic Field Acting Similarly to EGF Stimulation, Induces Acute Activations of the EGFR Sensitive Actin Cytoskeleton Motility in Human Amniotic Cells
Raus S et al. (2013): Response of hippocampal neurons and glial cells to alternating magnetic field in gerbils submitted to global cerebral ischemia
Saito A et al. (2009): Developmental effects of low frequency magnetic fields on P19-derived neuronal cells
Kroupova J et al. (2007): Low-frequency magnetic field effect on cytoskeleton and chromatin
Sul AR et al. (2006): Effects of sinusoidal electromagnetic field on structure and function of different kinds of cell lines
Tokalov SV et al. (2003): Comparison of the reactions to stress produced by X-rays or electromagnetic fields (50Hz) and heat: induction of heat shock genes and cell cycle effects in human cells
Wei M et al. (2000): Exposure to 60 Hz magnetic fields and proliferation of human astrocytoma cells in vitro

Acute and chronic effects of exposure to a 1-mT magnetic field on the cytoskeleton, stress proteins, and proliferation of astroglial cells in culture med./bio.

Aim of study (acc. to author)

Background/further details

Endpoint

Exposure

Exposure 1

Main characteristics

Exposure setup

Parameters

Exposure 2

Main characteristics

Exposure setup

Parameters

Exposure 3

Main characteristics

Exposure setup

Parameters

Exposure 4

Main characteristics

Exposure setup

Parameters

Methods Endpoint/measurement parameters/methodology

Main outcome of study (acc. to author)

Study funded by

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