Study type: Technical/dosimetric study (experimental study)

Acute and chronic effects of exposure to a 1-mT magnetic field on the cytoskeleton, stress proteins, and proliferation of astroglial cells in culture. med./bio.

Published in: Environ Res 2005; 98 (3): 355-362

Aim of study (acc. to author)

To examine the effects of exposure to magnetic fields on astroglial cells in culture.

Background/further details

1) The level of stress proteins HSP25, HSP60, and HSP70 and the cytoskeletal proteins actin and GFAP were determined after exposure to a 1-mT sinusoidal or static magnetic field or combined magnetic field for 1 hour. 2) The effect on the proteins after exposure to combined static/sinusoidal magnetic field for 1, 2, and 4 hours was examined. 3) The effect of a chronic (11-day) exposure to a combined magnetic field on the proliferation of cultured astroglial cells was investigated.

Endpoint

Exposure

Exposure Parameters
Exposure 1:
Exposure duration: continuous for 1 h
Exposure to DC field
Exposure 2: 50 Hz
Exposure duration: continuous for 1 h
Combination of AC and DC field
Exposure 3: 50 Hz
Exposure duration: continuous for 1 h, 2 h and 4 h
Combination of AC and DC field
Exposure 4: 50 Hz
Exposure duration: continuous for 11 days

Exposure 1

Main characteristics
Frequency
Type
Exposure duration continuous for 1 h
Additional info Exposure to DC field
Exposure setup
Exposure source
Setup pair of coils inside an incubator
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - measured - DC

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 1 h
Additional info Combination of AC and DC field
Additional info + DC of the same magnitude
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT effective value measured - AC for experiment 1
magnetic flux density 1 mT - measured - DC for experiment 2
magnetic flux density 1 mT effective value measured - AC + 1 mT DC for experiment 3

Exposure 3

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 1 h, 2 h and 4 h
Additional info Combination of AC and DC field
Additional info + DC of the same magnitude
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT effective value measured - AC
magnetic flux density 1 mT - measured - DC

Exposure 4

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 11 days
Additional info Chronic exposure
Additional info + DC of the same magnitude
Exposure setup
Exposure source
Setup field perpendicular to the flask base
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT effective value measured - AC
magnetic flux density 1 mT - measured - DC

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The results suggest that exposure to sinusoidal magnetic field, static magnetic field, or combined magnetic field (1 mT) has no significant effects on the level of cytoskeletal and stress proteins or on proliferation of astroglial cells in culture.

Study character:

Study funded by

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