Study type: Medical/biological study (experimental study)

Extremely low frequency electromagnetic field sensitizes cisplatin-resistant human ovarian adenocarcinoma cells via P53 activation. med./bio.

Published in: Cytotechnology 2016; 68 (4): 1403-1413

Aim of study (acc. to author)

It should be investigated if exposure to a 50 Hz magnetic field can overcome cisplatin resistance in human ovarian adenocarcinoma cells and initiate cell death at low concentrations of cisplatin.

Background/further details

Cells were divided into 6 groups: 1) treatment with 30 µg/ml cisplatin and exposure to the magnetic field, 2) 60 µg/ml cisplatin and MF exposure, 3) only MF exposure, 4) only treatment with 30 µg/ml cisplatin, 5) only treatment with 60 µg/ml cisplatin, 6) control group without exposure.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: for 2 hours

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration for 2 hours
Exposure setup
Exposure source
Setup after exposure, cells were returned to an incubator for further 46 hours
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 20 mT - - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

(remark EMF-Portal: significance of results becomes not clear and is contradictory in the article. Hence, no specifications on significance are reproduced in the following.)
Exposure to the magnetic field led to the reduction of cell viability in both co-exposure groups (groups 1 and 2) compared to exposure to the magnetic field alone (group 3), cisplatin alone (groups 4 and 5) or the control group (group 6).
Cells of group 1 showed a higher rate of DNA fragmentation, a higher amount of apoptotic cells and increased enzyme activities of caspases 3 and 9 compared to cells only exposed to the magnetic field (group 3), cisplatin (group 4) or the control group.
Gene expression of p53 was increased while gene expression of the matrix metalloproteinase-2 was decreased in group 1 compared to groups 3, 4 and 6.
The authors conclude that exposure to a 50 Hz magnetic field might sensibilize cisplatin-resistent human ovarian adenocarcinoma cells to low doses of cisplatin and thus enhance cell death.

Study character:

Study funded by

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