Study type: Medical/biological study (experimental study)

Effect of extremely low frequency of electromagnetic fields on cell proliferation, antioxidative enzyme activities and lipid peroxidation in 3T3-L1 preadipocytes--an in vitro study. med./bio.

Published in: J Physiol Pharmacol 2005; 56: 101-108

Aim of study (acc. to author)

To study the effects of extremely low frequency magnetic field on cell proliferation, antioxidative enzyme activities and malondialdehyde concentration in preadipocyte (precursor cells of adipocytes (lipocytes)) cell culture.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 180–195 Hz
Exposure duration: continuous for 36 min

Exposure 1

Main characteristics
Frequency 180–195 Hz
Type
Waveform
  • sawtooth-shaped
Exposure duration continuous for 36 min
Exposure setup
Exposure source
  • magnetostimulation device (VIOFOR JPS)
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 120 µT unspecified unspecified - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Extremely low frequency magnetic field exposure lasting 36 minutes daily failed to influence cell count after 24 h and 48 h of incubation.
After 24 h, in the magnetic field exposed group, manganese- and copper-zinc-containing superoxide dismutase enzyme activities were decreased, catalase activity was increased, whereas there were no significant differences in glutathione peroxidase and glutathione reductase activities in comparison to the control. After 48 h of incubation, all enzyme activities were reduced, except for glutathione reductase, in which no changes were found.
Malondialdehyde concentration at 24 h after incubation with the magnetic field exposure was significantly higher in comparison to the control. After 48 h of incubation, malondialdehyde levels were significantly lower in both groups (with no differences between the groups without and with magnetic field exposure).
The authors conclude that extremely low frequency magnetic field influences antioxidative enzyme activities and increases lipid peroxidation in these preadipocyte cultures.

Study character:

Study funded by

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