To study the effects of extremely low frequency electromagnetic fields and antioxidant supplementation (vitamin E), alone or in combination, on antioxidant enzyme activities and level of lipid peroxidation in a carcinoma cell line in vitro.
3 Hz–3 kHz
Exposure duration: continuous for 16 min
Following vitamin E treatment, antioxidant enzyme activity was increased compared with the untreated control group. Extremely low frequency electromagnetic field exposure alone or in combination with vitamin E increased both superoxide dismutase enzyme activities and glutathione peroxidase enzyme activity in comparison to the control group.
The data suggest that extremely low frequency electromagnetic fields alter antioxidative enzyme activities in AT478 tumor cells. This study confirms the role of vitamin E in decreasing susceptibility to lipid peroxidation (decreased levels of malondialdehyde) in AT478 tumor cells.
Further studies should be conducted to determine the influence of extremely low frequency electromagnetic field exposure and vitamin E on cell proliferation processes and chemosensitivity of the cancer cells to commonly used cytostatics causing reactive oxygen species-mediated damage (i.e. high levels of glutathione peroxidase in cancer cells lead to cancer cell survival via effective removal of hydrogen peroxide).