|Chamber||Two non-standard TEM cells (12 x 12 x 120 cm) were designed according to electromagnetic restraints and optimization of the uniform field volume [Van Hese et al., 1992]. They had a grid wall for air circulation. A water cooling system with two external metal jackets filled with circulating water fed from a thermostatic bath was placed in contact with the bottom walls of the TEM cell.|
|Setup||Mice restrained in ventilated transparent cylindrical Perspex jigs were positioned with their caudal axis parallel to the direction of the field propagation. The jigs were placed under and over the septum and kept at the same distance by polystyrene supports.|
|Sham exposure||A sham exposure was conducted.|
|Additional info||Eight mice at a time were placed in a TEM cell with daily clockwise rotation of their location to increase the homogeneity of exposure. The people preparing the biological samples and performing the biological tests did not know which TEM cell was activated. Control mice were maintained in the animal facility with minimal handling.|
Under the experimental conditions used, the data showed no effect of in vivo exposure to GSM modulated radiofrequency fields on B cell peripheral differentiation or on antibody production in response to both polyclonal activators and antigens. This indicates that it is unlikely that radiofrequency field exposure alters immune response to infections or vaccination.