研究のタイプ: 医学／生物学の研究 (experimental study)

[B細胞周辺分化と抗体産生に及ぼすGSM変調RF電磁界の影響] med./bio.

Effects of GSM-modulated radiofrequency electromagnetic fields on B-cell peripheral differentiation and antibody production

著者: Nasta F, Prisco MG, Pinto R, Lovisolo GA, Marino C, Pioli C
掲載誌: Radiat Res 2006; 165 (6): 664-670
RISフォーマットで参考文献をダウンロード

この研究は、マウスにGSM変調900 MHz RF電磁界の全身ばく露を与え、B細胞の末梢分化および抗体産生への影響を調べた。RFばく露は、全身平均SARが2 W / kgで、2時間/日で、連続4週間継続した。その結果、脾臓での分化の1型移行期（T1）および2型移行期（T2）B細胞の頻度にも、成熟濾胞B細胞や辺縁帯B細胞の頻度にもRFばく露は影響しなかった；IgMとIgGの血清レベルも、ばく露マウス、擬似ばく露マウス、および対照マウスの間で有意差はなかった；これらのマウスのB細胞に、インビトロでLPSの刺激を与えると、同等の量のIgMとIgGを産生した；さらに、免疫されたマウスにRF電磁界ばく露を与えても、抗原特異抗体の血清レベルは変化しなかった；抗原特異的なIgMの生成だけでなく、IgG（T-B細胞相互作用を必要とする）の生成もRF電磁界の影響を受けない；これは、RFばく露が体内で進行中の抗原特異的な免疫応答を変化させないことを示している、と報告している。

The detailed summary of this article is not available in your language or incomplete. Would you like to see a complete translation of the summary? Then please contact us →

研究目的（著者による）

To study the effects of in vivo exposure to a GSM-modulated 900 MHz radiofrequency field on B cell peripheral differentiation and antibody production in mice.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: pulsed
ばく露時間: repeated daily exposure, 2 h/day, 5 days/week, for 4 weeks
  • SAR: 2 W/kg mean (whole body)

General information

A detailed description of the exposure system and the dosimetry assessment has been published in [Ardoino et al., 2005].

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 repeated daily exposure, 2 h/day, 5 days/week, for 4 weeks
Modulation
Modulation type pulsed
Additional information

GSM basic modulation
ばく露装置
ばく露の発生源／構造
チャンバの詳細 Two non-standard TEM cells (12 x 12 x 120 cm) were designed according to electromagnetic restraints and optimization of the uniform field volume [Van Hese et al., 1992]. They had a grid wall for air circulation. A water cooling system with two external metal jackets filled with circulating water fed from a thermostatic bath was placed in contact with the bottom walls of the TEM cell.
ばく露装置の詳細 Mice restrained in ventilated transparent cylindrical Perspex jigs were positioned with their caudal axis parallel to the direction of the field propagation. The jigs were placed under and over the septum and kept at the same distance by polystyrene supports.
Sham exposure A sham exposure was conducted.
Additional information Eight mice at a time were placed in a TEM cell with daily clockwise rotation of their location to increase the homogeneity of exposure. The people preparing the biological samples and performing the biological tests did not know which TEM cell was activated. Control mice were maintained in the animal facility with minimal handling.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 2 W/kg mean 測定値および計算値 whole body -
測定および計算の詳細

The signal was continuously controlled by a bidirectional power sensor connected to a computer storing direct and reflected powers (and internal air temperature measurements). The EMF distribution was assessed using the numerical code HFSS (High Frequency Structure Simulator) and a calibrated E-field probe [Pioli et al., 2000]. The whole-body average SAR efficiency for eight mice in each TEM cell was determined numerically using two FDTD codes. Mice were assumed to be cylindrical (2.3 cm in diameter and 6 cm long) and homogeneous (modelling a 24-g mouse). The SAR values obtained were validated by means of a power balance method in mouse phantoms. Power measurements were also performed on live mice before, during and at the end of the experiments. The average efficiency value obtained for mice weighing 20-26 g was 0.38 W/kg/Win (SD 0.09) with a total uncertainty of about 20% (level of confidence 95%).

Reference articles

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

Under the experimental conditions used, the data showed no effect of in vivo exposure to GSM modulated radiofrequency fields on B cell peripheral differentiation or on antibody production in response to both polyclonal activators and antigens. This indicates that it is unlikely that radiofrequency field exposure alters immune response to infections or vaccination.

研究の種別:

研究助成

関連論文