Effects of 50 Hz sinusoidal magnetic fields on Hsp27, Hsp70, Hsp90 expression in porcine aortic endothelial cells (PAEC) med./bio.

[Wirkungen von sinusförmigen 50 Hz-Magnetfeldern auf die Hsp27-, Hsp70- und Hsp90-Expression in aortalen Endothelzellen des Schweins]

Ziel der Studie (lt. Autor)

Hintergrund/weitere Details

Endpunkt

• molekulare Biosynthese: Genexpression von Hsp27, Hsp70 und Hsp90

Exposition/Befeldung (teilweise nur auf Englisch)

• • 50/60 Hz
  • magnetisches Feld

Vollständige Ansicht Kompaktansicht

Methoden Endpunkt/Messparameter/Methodik

• molekulare Biosynthese: Genexpression von Hsp27, Hsp70 und Hsp90 (mRNA-Expression, Protein-Expression, Protein-Lokalisierung in den Zellen; RT-PCR-Quantifizierung, Western Blot, Immunofluoreszenz-Färbung)

Hauptergebnis der Studie (lt. Autor)

Studie gefördert durch

• Fondazione del Monte di Bologna e Ravenna, Italy
• Istituto Superiore per la Prevenzione e la Sicurezza del Lavoro (ISPESL; National Institute for Occupational Safety and Prevention), Italy
• Ministero dell' Università e della Ricerca (MIUR (formerly MURST (Ministero dell' Università e della Ricerca Scientifica e Tecnologica); Ministry of University and Research), Italy

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• Pipkin JL et al. (1999): Induction of stress proteins by electromagnetic fields in cultured HL-60 cells
• Goodman R et al. (1998): Magnetic field stress induces expression of hsp70
• Kang KI et al. (1998): Luciferase activity and synthesis of Hsp70 and Hsp90 are insensitive to 50Hz electromagnetic fields