Study type: Medical/biological study (experimental study)

Elliptically polarized magnetic fields do not alter immediate early response genes expression levels in human glioblastoma cells. med./bio.

Published in: Bioelectromagnetics 2002; 23 (2): 89-96

Aim of study (acc. to author)

To study the effects of different polarized magnetic fields on the immediate early genes expression in human glioblastoma cells in vitro.

Background/further details

Four types of polarization (vertical, horizontal, circular, elliptical) of the extremely low frequency magnetic field were performed and cells were exposed to different magnetic flux densities.
Positive controls were TPA treated cells.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Modulation type: CW
Exposure duration: 0.5; 1; 2; or 3 h
Exposure 2: 60 Hz
Modulation type: CW
Exposure duration: 0.5; 1; 2; or 3 h
Exposure 3: 60 Hz
Modulation type: CW
Exposure duration: 0.5; 1; 2; or 3 h
linear polarisation

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Polarization
  • elliptical
Exposure duration 0.5; 1; 2; or 3 h
Modulation
Modulation type CW
Exposure setup
Exposure source
Setup 4 coils per chamber, 2 horizontally and 2 vertically mounted, chambers surrounded by Mu metal box
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 500 µT maximum measured - -
magnetic flux density 1 µT minimum measured - -
magnetic flux density 20 µT - measured - -
magnetic flux density 100 µT - measured - -

Exposure 2

Main characteristics
Frequency 60 Hz
Type
Polarization
  • circular
Exposure duration 0.5; 1; 2; or 3 h
Additional info circular polarization
Modulation
Modulation type CW
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 500 µT - measured - -

Exposure 3

Main characteristics
Frequency 60 Hz
Type
Polarization
Exposure duration 0.5; 1; 2; or 3 h
Additional info linear polarisation
Modulation
Modulation type CW
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 500 µT - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The exposure to elliptically polarized magnetic fields up to two hours had no statistically significant effects on basal immediate early response gene and housekeeping gene expression. In addition, extremely low frequency magnetic field had no statistically significant effects on basal levels of expression of the immediate early genes and housekeeping genes in either polarisation type.
There was no significant difference in immediate early gene expression between magnetic field exposed and sham exposed cells at different magnetic flux densities of elliptically polarized magnetic fields.
The data do not provide any significant evidence that exposure to extremely low frequency magnetic fields of the human glioblastoma cell line affects immediate early response gene expression at either transcription or translation level.

Study character:

Study funded by

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