Study type: Medical/biological study (experimental study)

Evaluation of genotoxic effects in human leukocytes after in vitro exposure to 1950 MHz UMTS radiofrequency field. med./bio.

Published in: Bioelectromagnetics 2008; 29 (3): 177-184

Aim of study (acc. to author)

To study whether intermittent exposures at the frequency of 1950 MHz and UMTS signal are able to induce genotoxic effects in human blood cells.

Background/further details

For each donor (n=6) several experimental conditions were considered to check for a possible different sensitivity through the cell cycle (via micronucleus assay): (1) intermittent exposures of un-stimulated lymphocytes (resting cells) for 24 h, followed by 44 h exposure after phytohemagglutinin stimulation, to test radiofrequency exposure in G0 phase-G1 phase-S phase-G2 phase stages; (2) intermittent exposures for 44 h after phytohemagglutinin stimulation, to test radiofrequency exposure in G1 phase-S phase-G2 phase stages; (3) intermittent exposures of un-stimulated lymphocytes (resting cells) for 24 h before phytohemagglutinin stimulation. For comet assay un-stimulated lymphocytes (resting cells) were exposed for 24 h.
Positive controls were performed by treating cells with mitomycin C and methyl methanesulfonate for micronucleus and comet assays, respectively.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 1,950 MHz
Exposure duration: intermittent, 6 min on/2 h off, for 24, 44, or 24 + 44 h
  • SAR: 2.2 W/kg

Exposure 1

Main characteristics
Frequency 1,950 MHz
Type
Charakteristic
  • guided field
Exposure duration intermittent, 6 min on/2 h off, for 24, 44, or 24 + 44 h
Modulation
Modulation type cf. additional info
Additional info

UMTS 3GPP standard (FDD mode)

Exposure setup
Exposure source
Chamber The TEM cell was placed vertically inside an incubator equipped with air circulation systems.
Setup Two 25-ml Pyrex flasks filled with 10 ml of whole blood culture were placed 1 cm from each other on each side of the septum over a 1.5-mm thick Plexiglas shelf and radiated from the bottom.
Sham exposure A sham exposure was conducted.
Additional info Several independent temperature measurements were carried out in dummy cultures (flasks filled with culture medium) applying constant input power of 6 W for 6 min. A temperature increase of 0.67 ± 0.35 °C was measured, but the "off" period (2 h) was long enough to restore the initial conditions.
Parameters
Measurand Value Type Method Mass Remarks
SAR 2.2 W/kg - calculated - -

Reference articles

  • Zeni O et al. (2003): Lack of genotoxic effects (micronucleus induction) in human lymphocytes exposed in vitro to 900 MHz electromagnetic fields.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data showed that intermittent exposures in different stages of the cell cycle to the UMTS signal at SAR value of the current European safety limit do not induce genotoxic or cytotoxic effects in human lymphocytes.

Study character:

Study funded by

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