Peripheral blood samples were taken from six healthy non-smoking males aged beween 27 and 32 years. 2 x 250 randomly selected cells per treatment group were examined. Hydrogen peroxide treated cells were the positive controls.
The specific absorption rates tested were selected to evaluate conditions below (0.5 W/kg) or equal to (2 W/kg) the currently accepted safety limits by the ICNIRP.
For each donor, five conditions were tested in duplicate: RF and sham exposure at two SAR values and positive controls (cultures treated for 30 min with 50-µM hydrogen peroxide). The design of the exposure setup followed the basic requirements suggested in [Kuster et al., 2000].
|Exposure duration||continuous for 24 h|
|Setup||Four 35-mm Petri dishes filled with 3-ml samples were stacked in a plastic dish holder positioned inside the waveguide|
|Sham exposure||A sham exposure was conducted.|
|Additional info||induced electric field parallel to the sample surface, spatial distribution was quite uniform along the vertical axis; thus, a high uniformity was achieved between the different layers, including the cell layer at the bottom of the Petri dish. As already done in a previous work [Zeni et al., 2005], resonant operation was discarded in order to avoid limitations in the modulation bandwidth of the feeding signal.|