Study type: Medical/biological study (experimental study)

Induction of adaptive response in human blood lymphocytes exposed to 900 MHz radiofrequency fields: Influence of cell cycle. med./bio.

Published in: Int J Radiat Biol 2011; 87 (9): 993-999

Aim of study (acc. to author)

To study the influence of the cell cycle on the adaptive response induced by the exposure of human blood lymphocytes to radiofrequency fields.

Background/further details

Adaptive response: Different cell types that were exposed to an extremely small adaptation dose of a genotoxic agent were found to be less susceptible to the induction of genetic damage when given a higher challenge dose of the same or similar genotoxic agent. The induction of an adaptive response was shown to be influenced by several factors (e.g. the dose used for adaptation, the dose rate, the time between the adaptation and challenge doses).
Human peripheral blood lymphocytes in G0 phase, G1 phase or S phase of the cell cycle were exposed for 20 hours to an adaptive dose of 900 MHz radiofrequency and then treated with a challenge dose of 100 ng/ml mitomycin C.
Lymphocytes of nine different healthy male donors were investigated.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 900 MHz
Exposure duration: continuous for 20 h
  • SAR: 1.25 W/kg average over time
  • SAR: 10 W/kg peak value

Exposure 1

Main characteristics
Frequency 900 MHz
Type
Exposure duration continuous for 20 h
Exposure setup
Exposure source
Setup two wire patch cells placed inside an incubator with a constant temperature of 36.9° C +/- 0.5° C and a humidified atmosphere of 5% carbon dioxide and 95% air
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
SAR 1.25 W/kg average over time - - -
SAR 10 W/kg peak value - - -

Reference articles

  • Sannino A et al. (2009): Induction of adaptive response in human blood lymphocytes exposed to radiofrequency radiation.
  • Palumbo R et al. (2008): Exposure to 900 MHz radiofrequency radiation induces caspase 3 activation in proliferating human lymphocytes.
  • Scarfi MR et al. (2006): Exposure to radiofrequency radiation (900 MHz, GSM signal) does not affect micronucleus frequency and cell proliferation in human peripheral blood lymphocytes: an interlaboratory study.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data indicated that the cells which were exposed to an adaptive dose of radiofrequency in G0 phase and G1 phase of the cell cycle did not exhibit an adaptive response while such a response was observed when the cells were exposed to an adaptive dose of radiofrequency in the S phase of the cell cycle.
These findings confirmed the observations reported in previous investigations (Sannino et al. 2009) where an adaptive response was found in human blood lymphocytes exposed to an adaptive dose of radiofrequency in S phase of the cell cycle and further suggested that the timing of an adaptive dose exposure of radiofrequency is important to elicit an adaptive response.

Study character:

Study funded by

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