Study type: Medical/biological study (experimental study)

Human keratinocytes in culture exhibit no response when exposed to short duration, low amplitude, high frequency (900 MHz) electromagnetic fields in a reverberation chamber. med./bio.

Published in: Bioelectromagnetics 2011; 32 (4): 302-311

Aim of study (acc. to author)

Normal human epidermal keratinocytes were subjected to two non-thermal exposure conditions characterized by different amplitudes and durations to study possible changes in gene expression.

Background/further details

Eight microarrays (four exposed and four sham exposed) were screened per electromagnetic field condition.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 900 MHz
Modulation type: CW
Exposure duration: continuous for 10 min
Exposure 2: 900 MHz
Modulation type: CW
Exposure duration: continuous for 30 min

Exposure 1

Main characteristics
Frequency 900 MHz
Type
Exposure duration continuous for 10 min
Modulation
Modulation type CW
Exposure setup
Exposure source
Setup culture dishes individually enclosed in plastic boxes; eight boxes placed inside a temperature-controlled incubator made of 8 mm thick glas, internally insulated with 20 mm thick high-density polystyrene; incubator positioned in the working zone of a 8.4 m x 6.7 m x 3.5 m mode stirred reverberation chamber (MSRC) consisting of a metal-enclosed room; antenna pointed at a room's corner
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 8 V/m - measured - -
SAR 2.6 mW/kg - calculated - -

Exposure 2

Main characteristics
Frequency 900 MHz
Type
Exposure duration continuous for 30 min
Modulation
Modulation type CW
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 41 V/m - measured - -
SAR 73 mW/kg - calculated - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Under these conditions, exposure of keratinocytes to the electromagnetic field had only a small effect: only approx. 20 genes (out of 47000) displayed a significant alteration. Four genes were up-regulated and 15 down-regulated, but none were found under both exposure conditions. In addition, ratios of expression level were calculated and the ratios were small, ranging from 0.5 (down-regulation) to 1.6 (up-regulation). Furthermore, those assayed using RT-PCR (n=15 genes) did not display a significant expression modulation (i.e. no confirmation of the microarray data).
In conclusion, the data showed that cultured keratinocytes are not significantly affected by electromagnetic field exposure.

Study character:

Study funded by

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