研究のタイプ: 医学/生物学の研究 (experimental study)

[電磁界(900 MHz)はトマトに一定の分子反応をもたらす] med./bio.

Electromagnetic fields (900 MHz) evoke consistent molecular responses in tomato plants.

掲載誌: Physiol Plant 2006; 128 (2): 283-288

生物への高周波電磁界の影響が調べられているが、明確な結果が得られることは希である。これは適当に制御された研究が行われていないからである。ここでは、トマトを900MHzの電磁界ばく露し、ストレスに関連する転写カルモジュリンプロテアーゼ抑制、クロロプラストmRNA-結合タンパク)をPCRで測定した。電磁界へのばく露は2相的な反応を示し、3つすべての転写レベルが刺激終了後、15分で4-6倍ほど増加した。30分後には最初のレベルに低下し、60分後に増加した。

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研究目的(acc. to editor)

To study whether short exposure (10 minutes) of a plant to a low level radiofrequency electromagnetic field, similar to that used in mobile phones, could evoke a rapid biological response at the molecular level in tomato plants.

詳細情報

In order to determine if there was a dose response relationship between electromagnetic fields applied and the amount of transcript accumulated, plants were also exposed at others doses and durations.
Leaf tissue was harvested at 15, 30, and 60 minutes after exposure (in some experiments also immediately and after 5 minutes).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: CW
ばく露時間: continuous for 10 min
ばく露2: 900 MHz
Modulation type: CW
ばく露時間: continuous for 2 min

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 10 min
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
  • MSRC
チャンバの詳細 The MSRC (mode stirred reverberation chamber) consisted of a large room (200 m³) with a double-layered steel wall, acting as a Faraday cage, an antenna, and movable blades to stir the EMF. Tomato plants were grown inside plywood culture chambers. Shielded chambers were enclosed in a polymer mesh covered with aluminium foil to protect the plants from exposure [Vian et al., 2006].
ばく露装置の詳細 When the plants were 3 weeks old, the whole culture chamber was placed in the MSRC (24 h prior to irradiation) in a determined working volume where the field was homogeneous and isotropic.
Additional information The fourth terminal leaf (exposed and shielded) was harvested at different times after exposure (0, 5, 15, 30, and 60 min).
パラメータ
測定量 種別 Method Mass 備考
電界強度 5 V/m - - - average signal amplitude of a GSM phone
電界強度 40 V/m - - - close to the French legal emission limit

ばく露2

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 2 min
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
電界強度 5 V/m - - - average signal amplitude of a GSM phone

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Exposure to the electromagnetic fields induced a biphasic response, in which the levels of all three transcripts increased four- to six-fold 15 min. after the end of exposure, dropped closely to initial levels after 30 min., and then increased again at 60 min. after exposure. Although not identical, the kinetics and amplitudes of the transcripts showed striking similarities with physiologic responses following injurious treatments. Thus, the authors propose that radiofrequency electromagnetic field exposure may constitute an environmental stimulus for the tomato plants.

研究の種別:

研究助成

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