Study type: Medical/biological study (experimental study)

Cytogenetic effects of 18.0 and 16.5 GHz microwave radiation on human lymphocytes in vitro. med./bio.

Published in: Anticancer Res 2009; 29 (8): 2885-2892

Aim of study (acc. to author)

To study the cytogenetic effects of microwave irradiation alone or in combination with mitomycin C (co-exposure) in human lymphocytes.

Background/further details

Lymphocytes from two smoking and four non-smoking donors were exposed for 53 hours to continuous wave irradiation at 18.0 GHz or to pulsed wave at 16.5 GHz, alone or in combination with mitomycin C. In some cell cultures DNA synthesis (hydroxyurea) and DNA repair (caffeine) were inhibited.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 18 GHz
Modulation type: CW
Exposure duration: continuous for 53 hr
Exposure 2: 16.5 GHz
Modulation type: pulsed
Exposure duration: continuous for 53 hr

General information

cells were treated i) with RF exposure only ii) with RF exposure and mitomycin C after 30 hr of exposure

Exposure 1

Main characteristics
Frequency 18 GHz
Type
Charakteristic
Exposure duration continuous for 53 hr
Modulation
Modulation type CW
Exposure setup
Exposure source
Setup 5 m x 4 m x 4 m anechoic chamber with constant temperature and the horn antenna placed under the samples
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 19 V/m effective value measured - -
power density 1 W/m² average over time measured - -

Exposure 2

Main characteristics
Frequency 16.5 GHz
Type
Charakteristic
Exposure duration continuous for 53 hr
Modulation
Modulation type pulsed
Duty cycle 50 %
Repetition frequency 1 kHz
Exposure setup
Exposure source
Distance between exposed object and exposure source 1.2 m
Setup two rectangular horn antennas placed under the 5 m x 4 m x 4 m anechoic chamber with constant temperature
Parameters
Measurand Value Type Method Mass Remarks
power density 10 W/m² average over time measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No synergistic effect was observed in cells co-exposed to microwave irradiation and mitomycin C, or to cells pre-exposed in vivo to tobacco smoke. For the 16.5 GHz pulsed microwave exposure, a non-significant trend consisting of an increase in chromosome aberration frequencies with microwave irradiation was shown for the DNA synthesis and DNA repair inhibited cultures both with and without mitomycin C.
The authors conclude, that neither 18.0 GHz continuous wave nor 16.5 GHz pulsed wave exposure of human lymphocytes induced statistically significant increases in chromosome aberration frequencies. 16.5 GHz pulsed wave exposure requires further studies before a true negative conclusion can be drawn.

Study character:

Study funded by

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