Peripheral blood lymphocytes from nine different healthy donors were evaluated to investigate also the possible inter-individual response variability. Reproducibility was tested by repeating the experiment three months later.
|Exposure duration||continuous for 60, 120, and 180 min|
|Setup||Three millilitres of whole blood were exposed at room temperature (21-22 °C). Sample tubes were placed in a supporting frame, made of anechoic material, at an aerial distance ensuring continuous field conditions.|
|Additional info||Temperature variation was measured in water samples under the same experimental conditions of culture irradiation.|
It was shown that lymphocyte proliferation indices were significantly different among donors and between experiments, whereas the applied power density and the irradiation time did not have any effect on them.
Both spontaneous and induced micronuclei frequencies varied significantly among donors and between experiments. A statistically significant increase of micronuclei, although rather low, was revealed dependent on exposure time and applied power density. A considerable decrease in spontaneous and induced micronuclei frequencies was found in the second experiment.
The data show that microwaves are able to induce micronuclei in short-time exposures to medium power density fields. The data analysis highlights a wide inter-individual variability in the response, which was confirmed to be a characteristic reproducible trait by means of the second experiment.