Study type: Medical/biological study (experimental study)

No effect of 50 Hz 2.45 mT magnetic field on the potency of cisplatin, mitomycin C, and methotrexate in S. cerevisiae. med./bio.

Published in: Electromagn Biol Med 2008; 27 (3): 289-297

Aim of study (acc. to author)

To study whether a low frequency homogeneous magnetic field produces alterations of anti-cancer drugs potency in order to establish the basis for possible chemotherapy-sensitization by magnetic fields.

Background/further details

The following anti-cancer drugs were tested in yeast (Saccharomyces cerevisiae): cisplatin, mitomycin C and methotrexate (100 and 1000 µg/ml). Different experimental conditions were studied: 1) magnetic field exposure, 2) drug exposure, 3) magnetic field and drug exposure, and 4) un-exposed control group. Additionally, to assess the effects of temperature on cisplatin (1000 µg/ml), yeast cells were exposed to 20 °C, 27 °C and 30 °C.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 48 h

General information

cells were treated in four groups: i) exposed to MF ii) drug exposed iii) MF + drug exposed iv) unexposed control

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 48 h
Exposure setup
Exposure source
Setup pair of Helmholtz coils with a diameter of 40 cm, 20 cm apart, mounted on a wooden frame, connected in series; each coil consisting of 154 turn of 1.4 mm copper wire
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2.45 mT - measured - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Magnetic field exposure did not induce alterations in the potency of cisplatin, mitomycin C and methotrexate (at the doses assayed) on yeast cells in comparison to untreated control groups. There was no difference between magnetic field exposed groups and un-exposed control groups. A strong positive correlation between temperature and potency of cisplatin was found (importance of temperature control in experiments with anti-neoplastic agents in co-exposure with magnetic fields).

Study character:

Study funded by

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