Study type: Medical/biological study (experimental study)

Effect of 50 Hz magnetic field exposure on neuroblastoma morphology. med./bio.

Published in: Int J Integr Biol 2007; 1 (1): 12-17

Aim of study (acc. to author)

The effects of an acute in vitro exposure of neuroblastoma cells to a 50 Hz magnetic field on differentiation and proliferation processes should be investigated.

Background/further details

Cell growth tests were repeated three times. Neurofilaments are components of the neural cytoskeleton und NF160 and NF200 are two common types.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 5 days

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 5 days
Exposure setup
Exposure source
Chamber cells were placed in 5 ml Corning flasks in the exposure area
Setup solenoid consisting of a 2 cm thick, 20 cm high asbestos cylinder with a diameter of 20 cm and 1200 turns of 2 mm diameter copper wire wound in three layers in continuous forward-backward fashion; solenoid placed inside a cell incubator, the centre of which was ventilated by a fan to guarantee a constant temperature; magnetic field homogenuos within 5% in the 11 cm x 17 cm exposure area along the solenoid's axis
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2 mT effective value measured - -

Reference articles

  • Santoro N et al. (1997): Effect of extremely low frequency (ELF) magnetic field exposure on morphological and biophysical properties of human lymphoid cell line (Raji).
  • Stauffer PR et al. (1994): Practical induction heating coil designs for clinical hyperthermia with ferromagnetic implants

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

Cell growth was significantly decreased in exposed cell cultures compared to sham exposure (comment from EMF-Portal editorial office: the graph shows an increase in cell growth - a contradiction). Moreover, morphology of exposed cells was strikingly altered by a stretched appearance with axon-like structures and blebs. Finally, higher levels of neurofilaments NF160 and NF200 could be observed in exposed cells via immunofluorescence tests.
The authors conclude that the results indicate that an acute in vitro exposure of neuroblastoma cells to a 50 Hz magnetic field decreases their growth rate and changes the cell morphology towards a neuronal cell like form. However, these results were not evaluated in any form.

Study character:

Study funded by

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