Home
Medical/biological study (experimental study)

Chronic 835-MHz radiofrequency exposure to mice hippocampus alters the distribution of calbindin and GFAP immunoreactivity.

Published in: Brain Res 2010; 1346: 237-246

Aim of study (acc. to author)

To study the effect of 835 MHz radiofrequency exposure on mice hippocampus after three months of exposure.
Background/further details: Calcium binding proteins like calbindin D28k are responsible for the maintaining and controlling calcium homeostasis. Increased expression of glial fibrillary acidic protein (GFAP) has often been recognized in brain injury. Disturbance of Ca2+ homeostasis has been known to be Iinked with increase of GFAP immunoreactivity after brain injury.
20 mice were divided into two groups: sham exposure group and exposure group.

Endpoint

Exposure

    • RF field
    • CDMA
    • mobile communications
Exposure Parameters
Exposure 1: 835 MHz
Exposure duration: continuous for 8 hr/day for 3 months
Exposure 1
Main characteristics
Frequency 835 MHz
Type
Exposure duration continuous for 8 hr/day for 3 months
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 59.56 V/m - calculated - -
power 2.5 W - - - at 1.6 W/kg
SAR 1.6 W/kg average over time - - -
Reference articles
  • Maskey D et al. (2010): Effect of 835 MHz radiofrequency radiation exposure on calcium binding proteins in the hippocampus of the mouse brain.
Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated material:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data showed a decrease in calbindin D28k immune reactivity in the exposed group with loss of interneurons and pyramidal cells in CA1 area and loss of granule cells. Also, an overall increase in GFAP immune reactivity was observed in the hippocampus of exposed mice.
Apoptotic cells were detected in different areas of the hippocampus (CA1, CA3 and dentate gyrus) of exposed mice, which reflects that chronic radiofrequency exposure may affect the cell viability. In addition, the increase of GFAP immunoreactivity due to radiofrequency exposure could be related with reactive astrocytosis (abnormal increase in the number of astrocytes).
The data suggest that the decrease of calbindin D28k immune reactivity, accompanying apoptosis and increase of GFAP immune reactivity might be morphological parameters in the hippocampus damage.
Study character:

Study funded by

  • Dankook University, South Korea

Related articles

  • Razavinasab M et al. (2016): Maternal mobile phone exposure alters intrinsic electrophysiological properties of CA1 pyramidal neurons in rat offspring.
  • Erdem Koc G et al. (2016): Neuroprotective effects of melatonin and omega-3 on hippocampal cells prenatally exposed to 900 MHz electromagnetic fields.
  • Son Y et al. (2015): The Effect of Sub-Chronic Whole-Body Exposure to a 1,950 MHz Electromagnetic Field on the Hippocampus in the Mouse Brain.
  • Haghani M et al. (2013): Maternal mobile phone exposure adversely affects the electrophysiological properties of Purkinje neurons in rat offspring.
  • Maskey D et al. (2013): Neuroprotective effect of ginseng against alteration of calcium binding proteins immunoreactivity in the mice hippocampus after radiofrequency exposure.
  • Dogan M et al. (2012): Effects of electromagnetic radiation produced by 3G mobile phones on rat brains: Magnetic resonance spectroscopy, biochemical, and histopathological evaluation.
  • Watilliaux A et al. (2011): Effect of exposure to 1,800 MHz electromagnetic fields on heat shock proteins and glial cells in the brain of developing rats.
  • Finnie JW et al. (2010): Microglial activation as a measure of stress in mouse brains exposed acutely (60 minutes) and long-term (2 years) to mobile telephone radiofrequency fields.
  • Ammari M et al. (2010): GFAP expression in the rat brain following sub-chronic exposure to a 900 MHz electromagnetic field signal.
  • Maskey D et al. (2010): Effect of 835 MHz radiofrequency radiation exposure on calcium binding proteins in the hippocampus of the mouse brain.
  • Ait-Aissa S et al. (2010): In situ detection of gliosis and apoptosis in the brains of young rats exposed in utero to a Wi-Fi signal.
  • Sonmez OF et al. (2010): Purkinje cell number decreases in the adult female rat cerebellum following exposure to 900 MHz electromagnetic field.
  • Bas O et al. (2009): Chronic prenatal exposure to the 900 megahertz electromagnetic field induces pyramidal cell loss in the hippocampus of newborn rats.
  • Ragbetli MC et al. (2009): Effect of prenatal exposure to mobile phone on pyramidal cell numbers in the mouse hippocampus: a stereological study.
  • Bas O et al. (2009): 900 MHz electromagnetic field exposure affects qualitative and quantitative features of hippocampal pyramidal cells in the adult female rat.
  • Ammari M et al. (2008): Effect of a chronic GSM 900 MHz exposure on glia in the rat brain.
  • Kim TH et al. (2008): Local exposure of 849 MHz and 1763 MHz radiofrequency radiation to mouse heads does not induce cell death or cell proliferation in brain.
  • Odaci E et al. (2008): Effects of prenatal exposure to a 900 MHz electromagnetic field on the dentate gyrus of rats: a stereological and histopathological study.
  • Brillaud E et al. (2007): Effect of an acute 900MHz GSM exposure on glia in the rat brain: a time-dependent study.
  • Green AC et al. (2005): An investigation of the effects of TETRA RF fields on intracellular calcium in neurones and cardiac myocytes.
  • Mausset AL et al. (2001): Effects of radiofrequency exposure on the GABAergic system in the rat cerebellum: clues from semi-quantitative immunohistochemistry.
  • Paulraj R et al. (1999): Effect of amplitude modulated RF radiation on calcium ion efflux and ODC activity in chronically exposed rat brain.
  • Kunjilwar KK et al. (1993): Effect of amplitude-modulated radio frequency radiation on cholinergic system of developing rats.
  • Dutta SK et al. (1989): Radiofrequency radiation-induced calcium ion efflux enhancement from human and other neuroblastoma cells in culture.
  • Dutta SK et al. (1984): Microwave radiation-induced calcium ion efflux from human neuroblastoma cells in culture.
  • Merritt JH et al. (1982): Attempts to alter 45Ca2+ binding to brain tissue with pulse-modulated microwave energy.
  • Adey WR et al. (1982): Effects of weak amplitude-modulated microwave fields on calcium efflux from awake cat cerebral cortex.
  • Shelton Jr WW et al. (1981): In vitro study of microwave effects on calcium efflux in rat brain tissue.