Study type: Medical/biological study (experimental study)

Extremely low frequency magnetic fields induce oxidative stress in rat brain. med./bio.

Published in: Gen Physiol Biophys 2014; 33 (1): 81-90

Aim of study (acc. to author)

To investigate the influence of a long-term exposure to a 50 Hz-magnetic field on oxidative stress in the rat brain.

Background/further details

Three groups of rats were examined (n= 6 per group): 1.) sham exposure, 2.) exposure to a magnetic field of 50 µT and 3.) exposure to a magnetic field of 100 µT.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 90 days
Exposure 2: 50 Hz
Exposure duration: continuous for 90 days

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 90 days
Exposure setup
Exposure source
Chamber animals were housed individually, cages were covered with a stainless steel mesh lid
Setup exposure system consisted of 18 wooden bobbins (0.5 x 0.5 x 0.5 m³); wherein individual bobbins were wound by two sets of horizontal coils (each set of 25 turns); individual turns in the horizontal coil were seperated by a distance of 5 mm; coils were made of 22 gauge enameled copper wire; distance of 0.3 m seperated the individual bobbins to avoid interference
Sham exposure A sham exposure was conducted.
Additional info a set-up similar to the exposed animals was build for the sham exposed ones: 18 wooden bobbins were established without energizing the coils (2.78 m between exposed and sham exposed set-up).
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 50 µT - measured - -

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 90 days
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 100 µT - measured - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

In the hippocampus and the cerebellum of exposed groups (50 µT and 100 µT), the lipid peroxidation and enzyme activity of the glutathione peroxidase were significantly increased compared to the sham exposed group, while in the cortex only the 100 µT-exposed group showed a significant increase. In the hippocampus, cerebellum and cortex, the enzyme activity of the superoxide dismutase was significantly increased in the 100 µT-exposed group in comparison to the sham exposed group, but not in the 50 µT-group. The GSH/GSSG ratio was significantly decreased in all examined brain areas of the 100 µT-exposed group compared to the sham exposure group, while in the 50 µT-exposure group the GSH/GSSG ratio was only significantly decreased in the cerebellum.
No significant differences between the groups were seen regarding the body weight.
The authors conclude that long-term exposure to a 50 Hz-magnetic field induces oxidative stress in the rat brain.

Study character:

Study funded by

Related articles