研究のタイプ: 医学/生物学の研究 (experimental study)

[低周波磁界による強迫性障害的な行動への一酸化窒素の関与を示す実験的証拠] med./bio.

Experimental evidence for involvement of nitric oxide in low frequency magnetic field induced obsessive compulsive disorder-like behavior.

掲載誌: Pharmacol Biochem Behav 2014; 122: 273-278

この研究は、超低周波磁界(ELF MF)がヒトおよび実験動物神経系機能に影響を与えるという仮説に基づき、ELF MFが誘導する強迫性障害(OCD)のような行動へのドーパミンセロトニン一酸化窒素NO)の関与を動物実験で調べた。スイスアルビノマウスにELF MF(50Hz, 10G)へのばく露を1日8時間、7、30、60、90、120日間与えた。OCD様行動は、ガラス玉覆い隠し行動(marble-burying behavior:MBB)で評価した。その結果、ばく露日数依存性のMBBが誘導された;ELF MFばく露により、脳の各部位のNOのレベルは上昇したが、ドーパミンセロトニンのレベルは有意に変化しなかった、などを報告している。

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研究目的(著者による)

To investigate the involvement of dopamine, serotonin and nitric oxide in extremely low frequency magnetic field-induced obsessive compulsive disorder-like behavior in mice.

詳細情報

Three experiments were performed:
In the first experiment, several groups of mice (n=8 per group) were subjected to behavioral tests. They were exposed or sham exposed for 7, 30, 60, 90 or 120 days. For the examination of the obsessive compulsive disorder-like behavior and for the determination of the locomotor activity, separate groups were employed.
In the second experiment, the levels of dopamine, serotonin and nitric oxide in the brain were determined after 120 days of sham exposure (n=8) or exposure (n=8).
In the third experiment, the influence of different nitric oxide modulators on behavior and levels of dopamine, serotonin and nitric oxide in the brain were examined. Mice were exposed or sham exposed for 120 days and received daily injections of saline solution (as a control), L-NAME (inhibitor of nitric oxide synthase; 15, 30 or 45 mg per kg body weight), L-ARG (nitric oxide precursor; 200, 400 or 800 mg per kg body weight) or 7-NI (inhibitor of nitric oxide synthase; 10, 20 or 40 mg per kg body weight) before the exposure/sham exposure. Separate groups (n=8 per group) were employed for each dose and examination.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: 8 hours/day for 7, 30, 60, 90 or 120 days

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 8 hours/day for 7, 30, 60, 90 or 120 days
ばく露装置
ばく露の発生源/構造
チャンバの詳細 mice were housed in a clean plastic cage which was positioned on the center of the platform of the coils
ばく露装置の詳細 Helmholtz coils, spaced apart at a distance equal to their radii (45 cm); instrument was constructed by winding glaze-insulated copper wire (d = 1.2 mm) 100 times on wooden frames; both the coils were individually attached to regulated AC power supply through step down transformer (12 V) which acted as an isolator
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT - 測定値 - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The first experiment revealed that magnetic field exposed mice buried significant more marbles than sham exposed mice. Furthermore, this effect was time dependent: The longer the mice were exposed the more marbles were buried and the more significant was the result. No significant differences between any of the groups were found regarding locomotor activity.
In the second experiment, no significant effect of the exposure on the levels of dopamine and serotonin in the brain of the mice was observed. However, exposure led to significantly increased nitric oxide levels in the cortex, hippocampus and hypothalamus when compared to the corresponding sham exposure.
In the third experiment, it was shown that the combination of the magnetic field exposure and the nitric oxide precursor L-ARG led to a further significant increase in the number of buried marbles compared to the magnetic field exposure alone, while the nitric oxide synthase inhibitors L-NAME and 7-NI significantly attenuated the exposure induced effect.
The authors conclude that exposure to extremely low frequency magnetic fields could induce obsessive compulsive disorder-like behavior in mice and that this effect appears to be associated with nitric oxide synthase activation.

研究の種別:

研究助成

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