32 male rats were divided into four groups: 1) control group, 2) exposure group, 3) immobilization, 4) exposure + immobilization.
Immobilization was used as a positive control for physical and psychological stress.
Exposure duration: continuous for 2 h
|Setup||rats were restrained by confinement into a 18 cm long acrylic cylinder with an inner diameter of 7 cm; unrestained rats were placed individually in 47 cm x 25 cm x 21 cm acrylic cages; pair of circular Helmholtz coils with an inner diameter of 30 cm, consisting of 350 turns of 18-gauge copper wire; coils separated by 15 cm from the upper and lower surfaces of the animal cage|
|Sham exposure||A sham exposure was conducted.|
|magnetic flux density||2.4 mT||-||measured||-||-|
Acute exposure to extremely low frequency magnetic field alone as well as "exposure + immobilization" induced a reduction in catalase and superoxide dismutase enzyme activities compared with the control group, whereas the combination of "magnetic field exposure and immobilization" also decreased both reduced glutathione (compared with the control group) and nitric oxide levels compared with the other groups. Thiobarbituric acid reactive substances in the total lipid content of the brain were higher in the "exposure + immobilization group" than in the other experimental groups. Exposure to extremely low frequency magnetic fields did not induce any changes of the stress-hormone corticosterone or triacylglycerol levels.
Acute exposure to the extremely low frequency magnetic field appears to be a mild stressor.