Study type: Medical/biological study (experimental study)

50 Hz electromagnetic field exposure promotes proliferation and cytokine production of bone marrow mesenchymal stem cells. med./bio.

Published in: Int J Clin Exp Med 2015; 8 (5): 7394-7404

Aim of study (acc. to author)

The effects of exposure of mesenchymal stem cells from rat and mouse to a 50 Hz magnetic field on cell proliferation and cytokine production should be investigated.

Background/further details

Rat and mouse stem cells were divided into two groups, respectively: 1) exposure to the magnetic field and 2) sham exposure (remark EMF-Portal: additional tests were conducted with macrophages, but as they did not investigate the direct effects of exposure to the magnetic field, they are not regarded here).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 4 hours/day for up to 7 days

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 4 hours/day for up to 7 days
Exposure setup
Exposure source
Chamber petry dishes, well plates and flasks (depending on method)
Setup two independent electromagnetic field generators connected to a computer via a specific sensor
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - - - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • before exposure
  • after exposure

Main outcome of study (acc. to author)

The following parameters were significantly increased in exposed rat stem cells compared to the sham exposure group: cell proliferation after 3 to 7 days, DNA synthesis, proportion of cells in S phase in the cell cycle distribution and the gene expression rates of SCF and CSF after 2 and 3 days.
In mouse stem cells, the gene expression rates of SCF, CSF, TPO, LIF, IL-11 and IL-7 were significantly increased in the exposure group compared to the sham exposure group.
The authors conclude that exposure of mesenchymal stem cells from rat and mouse to a 50 Hz magnetic field could stimulate the cell proliferation and the production of some cytokines.

Study character:

Study funded by

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