50 Hz electromagnetic field exposure promotes proliferation and cytokine production of bone marrow mesenchymal stem cells.
Published in: Int J Clin Exp Med 2015; 8 (5): 7394-7404
Aim of study (acc. to author)
continuous for 4 hours/day for up to 7 days
- molecular biosynthesis: DNA synthesis in rat stem cells (after 3 days; incorporation of 5-ethynyl-2'-deoxyuridine (EdU), fluorescence microscopy), gene expression of cytokines in rat (after 0, 1, 2 and 3 days; SCF, CSF) and mouse (after 3 days; SCF, CSF, TPO, LIF, IL-11, IL-7, IL-6, SDF-1, IFN-gamma and TNF-alpha) stem cells (real-time RT-PCR)
- cell viability/cell division/proliferation: cell proliferation of rat stem cells (after 0, 1, 3, 5 and 7 days; commercial kit, spectrophotometry); cell cycle distribution of rat stem cells (after 3 days; flow cytometry)
Time of investigation:
- before exposure
- after exposure
Main outcome of study (acc. to author)
Study funded by
National Basic Research Program (Program 973), China
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