研究のタイプ: 医学/生物学の研究 (experimental study)

[50Hzの電磁界ばく露は骨髄間葉系幹細胞の増殖およびサイトカイン産生を促進する] med./bio.

50 Hz electromagnetic field exposure promotes proliferation and cytokine production of bone marrow mesenchymal stem cells.

掲載誌: Int J Clin Exp Med 2015; 8 (5): 7394-7404

この研究は、ラット骨髄由来間葉幹細胞(rBMSC)の増殖およびサイトカイン産生に対する超低周波磁界(ELF:50Hz、1mT、1日4時間、3日間)ばく露の影響、およびELFばく露下で3日間培養したマウスの間葉系幹細胞培養上清(mMSC-CM)の投与によるマクロファージ(RAW264.7)の増殖移動への影響を調べた。その結果、ELFばく露はrBMSCの増殖を上昇させた:ELFばく露したmMSC-CMを投与したRAW264.7の増殖移動が増加した、と報告している。

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研究目的(著者による)

The effects of exposure of mesenchymal stem cells from rat and mouse to a 50 Hz magnetic field on cell proliferation and cytokine production should be investigated.

詳細情報

Rat and mouse stem cells were divided into two groups, respectively: 1) exposure to the magnetic field and 2) sham exposure (remark EMF-Portal: additional tests were conducted with macrophages, but as they did not investigate the direct effects of exposure to the magnetic field, they are not regarded here).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 4 hours/day for up to 7 days

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 4 hours/day for up to 7 days
ばく露装置
ばく露の発生源/構造
  • electromagnetic field generator
チャンバの詳細 petry dishes, well plates and flasks (depending on method)
ばく露装置の詳細 two independent electromagnetic field generators connected to a computer via a specific sensor
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT - - - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露前
  • ばく露後

研究の主なアウトカム(著者による)

The following parameters were significantly increased in exposed rat stem cells compared to the sham exposure group: cell proliferation after 3 to 7 days, DNA synthesis, proportion of cells in S phase in the cell cycle distribution and the gene expression rates of SCF and CSF after 2 and 3 days.
In mouse stem cells, the gene expression rates of SCF, CSF, TPO, LIF, IL-11 and IL-7 were significantly increased in the exposure group compared to the sham exposure group.
The authors conclude that exposure of mesenchymal stem cells from rat and mouse to a 50 Hz magnetic field could stimulate the cell proliferation and the production of some cytokines.

研究の種別:

研究助成

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