Medical/biological study (experimental study)

Effects of prenatal exposure to extremely low electro - magnetic field on in vivo derived blastocysts of mice.

Published in: Int J Reprod Biomed (Yazd) 2012; 10 (6): 555-560

Aim of study (acc. to author)

To investigate the possible effects of exposure to 50 Hz magnetic fields during early pregnancy on the number and quality of mice blastocysts.
Background/further details: In total, 66 mice were divided into an exposure and a control group (n=33, respectively). Blastocysts were obtained by flushing the uterine horn and fallopian tubes on day three of gestation.



Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuously for 48 hours
Exposure 1
Main characteristics
Frequency 50 Hz
Exposure duration continuously for 48 hours
Exposure setup
Exposure source
Setup exposure system consisted of a power supply with a permanent current intensity of 3 A, 220 V input and 5 V output voltage, a multi-meter to control the intensity of the current and the magnetic field generating device; the Helmholtz coil with 380 turns wound around a cylinder (diameter 19 cm, length 15.5 cm) contained a chamber to house the mice in the center of the cylinder; temperature was 37°C ± 0.1°C
Measurand Value Type Method Mass Remarks
magnetic flux density 6 mT - measured - ± 0.1 mT
Exposed system:
  • animal
  • mouse/NMRI
  • whole body

Methods Endpoint/measurement parameters/methodology

Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No significant difference was found in the number of blastocysts between the exposure and the control group. However, the number of total cells, trophoblast cells, and embryoblast cells were significantly reduced in blastocysts from exposed animals compared to those derived from the control group while the ratio of embryoblast cells to trophoblast cells was significantly increased.
The authors conclude that 50 Hz magnetic field exposure could affect the cellular composition of blastocysts in pregnant mice and could hence influence the fertility of female mice.
Study character:

Study funded by

  • Arak University of Medical Sciences, Iran

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