Study type: Medical/biological study (experimental study)

Analysis of DNA fragmentation in mouse embryos exposed to an extremely low-frequency electromagnetic field. med./bio.

Published in: Electromagn Biol Med 2011; 30 (4): 246-252

Aim of study (acc. to author)

To study the possible effects of electromagnetic field exposure on DNA fragmentation in the blastomeres of mouse blastocysts.

Background/further details

80 female mice were divided into two groups of 40 animals each: 1) control group and 2) exposure group. After the 8th day of exposure, the female mice in both groups were superovulated (with injections of gonadotropin) and then mated overnight. At approximately four days after mating blastocysts were obtained by flushing the uterus horns. 50 blastocysts were obtained from the controls and 60 blastocysts from the exposed group.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 4 h/day, 6 days/week for 2 weeks

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 4 h/day, 6 days/week for 2 weeks
Exposure setup
Exposure source
Setup pair of 30 cm x 30 cm square-shaped Helmholtz coils mounted in two frames; each coil with 1000 turns of copper wire; 27 cm x 21 cm x 14 cm cage with five animals placed on a platform between the coils; exposure system placed on a laboratory non-metallic table
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.5 mT - calibration - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data showed that the mean number of blastocysts was significantly decreased in the exposed group compared with the control group. The DNA fragmentation index was significantly increased in the exposed group compared with the control. However, there was no significant difference in the mean numbers of blastomeres and numbers of pregnant mice between the exposed and control group.
The findings indicate that the electromagnetic field exposure in preimplantation stage could have detrimental effects on female mouse fertility and embryo development by decreasing the number of blastocysts and increasing the blastocysts DNA fragmentation.

Study character:

Study funded by

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