80 female mice were divided into two groups of 40 animals each: 1) control group and 2) exposure group. After the 8th day of exposure, the female mice in both groups were superovulated (with injections of gonadotropin) and then mated overnight. At approximately four days after mating blastocysts were obtained by flushing the uterus horns. 50 blastocysts were obtained from the controls and 60 blastocysts from the exposed group.
Exposure duration: continuous for 4 h/day, 6 days/week for 2 weeks
|Exposure duration||continuous for 4 h/day, 6 days/week for 2 weeks|
|Setup||pair of 30 cm x 30 cm square-shaped Helmholtz coils mounted in two frames; each coil with 1000 turns of copper wire; 27 cm x 21 cm x 14 cm cage with five animals placed on a platform between the coils; exposure system placed on a laboratory non-metallic table|
|Sham exposure||A sham exposure was conducted.|
|magnetic flux density||0.5 mT||-||calibration||-||-|
The data showed that the mean number of blastocysts was significantly decreased in the exposed group compared with the control group. The DNA fragmentation index was significantly increased in the exposed group compared with the control. However, there was no significant difference in the mean numbers of blastomeres and numbers of pregnant mice between the exposed and control group.
The findings indicate that the electromagnetic field exposure in preimplantation stage could have detrimental effects on female mouse fertility and embryo development by decreasing the number of blastocysts and increasing the blastocysts DNA fragmentation.