Medical/biological study (experimental study)

Effects of extremely low-frequency electromagnetic field on fertility and heights of epithelial cells in pre-implantation stage endometrium and fallopian tube in mice med./bio.

By: Rajaei F, Borhani N, Sabbagh-Ziarani F, Mashayekhi F

Published in: Zhong Xi Yi Jie He Xue Bao 2010; 8 (1): 56-60

Aim of study (acc. to author)

To study the effects of an extremely low frequency electromagnetic field (50 Hz, 0.5 mT) on the fertility and heights of epithelial cells in pre-implantation stage in the endometrium and fallopian tube in mice.

Background/further details

80 female rats were randomly divided into two groups: control group and exposure group. At the time of implantation, the blastocysts were obtained from the presumed pregnant mice (controlled by vaginal plug) by flushing the isolated (dissected) uterus horns.

Endpoint

effects on the reproductive system: fertility; effects on the number of blastocysts
morphol./histopathol. changes: height of endometrial and of fallopian tube epithelial cells

Exposure

- source not given

Exposure	Parameters
Exposure 1: 50 Hz Exposure duration: continuous for 4 hr/day, 6 days/week for 2 weeks	magnetic flux density: 0.5 mT

Exposure 1

Main characteristics

Frequency	50 Hz
Type	magnetic field
Exposure duration	continuous for 4 hr/day, 6 days/week for 2 weeks

Exposure setup

Exposure source	not specified

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	0.5 mT	-	-	-	-

Exposed system:

animal
mouse/NMRI
whole body

Methods Endpoint/measurement parameters/methodology

effects on the reproductive system: fertility; effects on the number of blastocysts
morphol./histopathol. changes: height of endometrial and of fallopian tube epithelial cells (hematoxylin-eosin stain; light microscopy)

Investigated system:

tissue slices
isolated organ
flushed blastocysts

Investigated organ system:

reproductive system

Time of investigation:

after exposure

Main outcome of study (acc. to author)

The analysis of the mean number of the flushed blastocysts in the exposed group showed a significant decrease as compared with the control group. The height of the fallopian tube epithelial cells was significantly increased in the exposed group as compared with the control group. However the height of the endometrial epithelial cells in the electromagnetic field exposed group showed insignificant increase as compared with the control group.
The authors conclude that an extremely low frequency electromagnetic field exposure has a detrimental effect on the female reproductive system in mice by decreasing the number of flushed blastocysts and increasing the height of fallopian tube epithelial cells.

Study character:

medical/biological study
experimental study
full/main study

Study funded by

Qazvin University of Medical Sciences, Iran

Burcu A et al. (2020): The effects of prenatal and postnatal exposure to electromagnetic field on rat ovarian tissue
Franczak A et al. (2020): Consequences of electromagnetic field (EMF) radiation during early pregnancy - androgen synthesis and release from the myometrium of pigs in vitro
Kim HS et al. (2014): Continuous exposure to 60 Hz magnetic fields induces duration- and dose-dependent apoptosis of testicular germ cells
Roshangar L et al. (2014): Effect of low-frequency electromagnetic field exposure on oocyte differentiation and follicular development
Bayat PD et al. (2012): Effects of prenatal exposure to extremely low electro - magnetic field on in vivo derived blastocysts of mice
Borhani N et al. (2011): Analysis of DNA fragmentation in mouse embryos exposed to an extremely low-frequency electromagnetic field
de Bruyn L et al. (2010): Effect of long-term exposure to a randomly varied 50 Hz power frequency magnetic field on the fertility of the mouse
Kim YW et al. (2009): Effects of 60 Hz 14 microT magnetic field on the apoptosis of testicular germ cell in mice
Pourlis AF (2009): Reproductive and developmental effects of EMF in vertebrate animal models
Aydin M et al. (2009): Evaluation of hormonal change, biochemical parameters, and histopathological status of uterus in rats exposed to 50-Hz electromagnetic field
Roychoudhury S et al. (2009): Influence of a 50 Hz extra low frequency electromagnetic field on spermatozoa motility and fertilization rates in rabbits
Rajaei F et al. (2009): Effects of extremely low-frequency magnetic field on mouse epididymis and deferens ducts
Al-Akhras MA (2008): Influence of 50 Hz magnetic field on sex hormones and body, uterine, and ovarian weights of adult female rats
Roushangar L et al. (2007): Ultrastructural alterations and occurrence of apoptosis in developing follicles exposed to low frequency electromagnetic field in rat ovary
Robert E (1996): Teratogen update: electromagnetic fields