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The cells were divided into the following groups: 1) exposure to a 60 Hz magnetic field with 2 - 6 mT, 2) 1) exposure to a 120 Hz magnetic field with 2 - 6 mT, 3) exposure to a 60 Hz magnetic field with 2 - 6 mT and subsequent further incubation without exposure for 24 hours, 4) exposure to a 120 Hz magnetic field with 2 - 6 mT and subsequent further incubation without exposure for 24 hours. A separate control group was used for each exposure condition.
In all groups, the proportion of cells in the G0/G1 phase was significantly increased compared to the respective control group, while the proportion of cells in the S phase was significantly reduced. The effects of the 60 Hz magnetic fields (groups 1 and 3) were significantly stronger compared to the 120 Hz groups (groups 2 and 4). (Note EMF-Portal: No information was provided on the magnetic flux density used in cell cycle progression experiments.) These results indicated an anti-proliferative effect of magnetic field exposure.
Cell viability was significantly increased in all groups and at all magnetic flux densities compared to the control groups.
The authors conclude that exposure of pig anterior pituitary cells to a 60 Hz or 120 Hz magnetic field could have an anti-proliferative effect by influencing cell cycle progression.