Study type: Medical/biological study (experimental study)

Morphometric evaluation of Japanese quail embryos and their extraembryonic vascular networks exposed to low-frequency magnetic field with two different intensities. med./bio.

Published in: Electromagn Biol Med 2020; 39 (4): 403-410

Aim of study (acc. to author)

The effects of exposure of Japanese quail embryos to a 60 Hz magnetic field on the development of the embryonic body and vascular network of the yolk sac should be investigated.

Background/further details

A total of 30 eggs of Japanese quail were divided into the following groups (n=10, respectively): 1) exposure to a 0.16 mT magnetic field, 2) exposure to a 0.65 mT magnetic field, 3) control group. After 24 h of egg incubation, an opening of approximately 3 cm2 was made with sterile scissors and it was covered with parafilm. This opening allowed the embryo and yolk sac membrane to be visualized. Exposure to the magnetic field startet at 54 h of incubation and finished exactly at 72 h.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: 3 x 2 hours, with 6-hour intervals in between resulting in a total exposure of 6 h
Exposure 2: 60 Hz
Exposure duration: 3 x 2 hours, with 6-hour intervals in between resulting in a total exposure of 6 h

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Exposure duration 3 x 2 hours, with 6-hour intervals in between resulting in a total exposure of 6 h
Exposure setup
Exposure source
Setup eggs were placed in an incubator (temperature of 37.5°C and humidity above 60%) associated with a pair of Helmholtz coils
Sham exposure A sham exposure was conducted.
Additional info eggs of the control group were incubated in the same incubator associated with the Helmholtz coils turned off
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.16 mT - measured - -

Exposure 2

Main characteristics
Frequency 60 Hz
Type
Exposure duration 3 x 2 hours, with 6-hour intervals in between resulting in a total exposure of 6 h
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.65 mT - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Embryos exposed to the 0.65 mT magnetic field (group 2) showed a significantly smaller body area and percentage cephalic length compared to the control group.
The authors concluded that exposure of Japanese quail embryos to a 60 Hz magnetic field showed no effects on vascular network of the yolk sac but might decrease the development of the embryonic body.

Study character:

Study funded by

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