To determine whether exposure of NGF (nerve growth factor)-treated PC12 cells to an athermal modulated radiofrequency field at 836.55 MHz altered the expression of the immediate early genes encoding c-jun and c-fos.
NGF is required for the survival and development of neuronal populations in both the central and peripheral nervous system, and it influences the phenotypic characteristics of neurons in vivo and in vitro.
Modulation type: pulsed
Exposure duration: intermittent, 20 min on/off, for 20, 60 and 100 min
|Chamber||Two TEM cells of square cross section, one powered and one for sham exposure, were placed in a water-jacketed incubator maintained at 37 °C and 5% CO2.|
|Setup||Cells were seeded on 100 mm Petri dishes with 20 ml of medium (depth of 3 mm). In most experiments, two stacks of two dishes each were placed centrally on the septum in each TEM cell, and in a few experiments, dishes were placed on both the septum and the bottom of the TEM cells in an identical fashion. The E-field was normal to the Petri dishes.|
|Additional info||The transmitter was switched on/off at 20 min intervals for total exposures of 20, 40, and 60 min (20, 60, and 100 min total incubation times).|
|power||0.17 W||average over time||measured||-||0.017, 0.17 and 1.7 W|
|power density||0.3 mW/cm²||average over time||measured||-||0.03, 0.3 and 3 mW/cm²|
|power density||0.9 mW/cm²||cf. remarks||measured||-||0.09, 0.9 and 9 mW/cm² slot average|
|SAR||2.6 µW/g||cf. remarks||calculated||-||± 1.9 µW/g slot average at 0.9 mW/cm²|
No change in c-fos transcript levels were detected after 20 min exposure at each field intensity (20 min was the only time period at which the c-fos transcript could be detected consistently). Transcript levels for c-jun were altered only after 20 min exposure (9mW/cm²).