Study type: Medical/biological study (experimental study)

Analysis of proto-oncogene and heat-shock protein gene expression in human derived cell-lines exposed in vitro to an intermittent 1.9 GHz pulse-modulated radiofrequency field. med./bio.

Published in: Int J Radiat Biol 2006; 82 (5): 347-354

Aim of study (acc. to author)

To study the effects of radiofrequency fields, emitted by mobile phones, on gene expression in cultured human cell lines at 0 and 18 h after exposure.

Background/further details

All samples were compared to sham-exposed, negative (incubator) and positive (heat shock, 1 h at 43°C) controls at 0 and 18 h after exposure.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 1.9 GHz
Modulation type: pulsed
Exposure duration: intermittent, 5 min on/10 min off, for 6 h

Exposure 1

Main characteristics
Frequency 1.9 GHz
Type
Polarization
  • circular
Exposure duration intermittent, 5 min on/10 min off, for 6 h
Modulation
Modulation type pulsed
Exposure setup
Exposure source
Chamber The temperature in the cell cultures was maintained at 37.0 ± 0.5°C.
Setup Cells were exposed in 10 ml of culture medium in 60-mm Petri dishes placed atop circularly polarized cylindrical waveguide applicators.
Sham exposure A sham exposure was conducted.
Additional info Negative and positive (heat shock) controls were run concurrently with each experiment.
Parameters
Measurand Value Type Method Mass Remarks
SAR 1 W/kg mean unspecified - ±24%
SAR 10 W/kg mean unspecified - ±24%

Reference articles

  • Gajda GB et al. (2002): Cylindrical waveguide applicator for in vitro exposure of cell culture samples to 1.9-GHz radiofrequency fields.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No significant effects were observed in mRNA expression of hsp27, hsp70, c-jun, c-myc or c-fos in comparison of the sham-exposed and radiofrequency-exposed groups, in either of the two cell lines. However, the positive control displayed a significant elevation in the expression of hsp27, hsp70, c-fos and c-jun in both cell lines compared to the sham-exposed and negative control groups.
The data revealed no evidence that exposure of cells to non-thermal levels of 1.9 GHz pulse modulated radiofrequency fields can cause any detectable change in stress-related gene expression.

Study character:

Study funded by

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