Study type: Medical/biological study (experimental study)

Analysis of gene expression in two human-derived cell lines exposed in vitro to a 1.9 GHz pulse-modulated radiofrequency field. med./bio.

Published in: Proteomics 2007; 7 (21): 3896-3905

Aim of study (acc. to author)

As a follow-up study of publication 13899, the authors examined the effect of radiofrequency electromagnetic field exposure on gene expression in cells (U87MG) after a 24 h radiofrequency exposure period.

Background/further details

In addition, human Mono Mac 6 cells were exposed to intermittent radiofrequency electromagnetic fields for 6 h and then gene expression was assessed immediately and at 18 h after exposure. Both cell lines were exposed to 1.9 GHz pulse modulated radiofrequency fields for 6 or 24 h at different specific absorption rates.
Positive controls were performed at 43°C (1 h).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 1.9 GHz
Modulation type: pulsed
Exposure duration: continuous for 24 h
U87MG cells
Exposure 2: 1.9 GHz
Modulation type: pulsed
Exposure duration: intermittent, 5 min on/10 min off, for 6 h
MM6 cells

Exposure 1

Main characteristics
Frequency 1.9 GHz
Type
Charakteristic
  • guided field
Polarization
  • circular
Exposure duration continuous for 24 h
Additional info U87MG cells
Modulation
Modulation type pulsed
Duty cycle 33.33 %
Repetition frequency 50 Hz
Exposure setup
Exposure source
Setup Six circularly polarized cylindrical waveguides were used to expose the cell culture samples in 60-mm culture dishes.
Sham exposure A sham exposure was conducted.
Additional info Temperature within the cell cultures was monitored at 60-s intervals using non-perturbing thermistor probes, as described in [Qutob et al., 2006].
Parameters
Measurand Value Type Method Mass Remarks
SAR 0.1 W/kg mean - - ±24%
SAR 1 W/kg mean - - ±24%
SAR 10 W/kg mean - - ±24%

Exposure 2

Main characteristics
Frequency 1.9 GHz
Type
Charakteristic
  • guided field
Polarization
  • circular
Exposure duration intermittent, 5 min on/10 min off, for 6 h
Additional info MM6 cells
Modulation
Modulation type pulsed
Duty cycle 33.33 %
Repetition frequency 50 Hz
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
SAR 1 W/kg mean - - ±24%
SAR 10 W/kg mean - - ±24%

Reference articles

  • Qutob SS et al. (2006): Microarray gene expression profiling of a human glioblastoma cell line exposed in vitro to a 1.9 GHz pulse-modulated radiofrequency field.
  • Gajda GB et al. (2002): Cylindrical waveguide applicator for in vitro exposure of cell culture samples to 1.9-GHz radiofrequency fields.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • before exposure
  • after exposure

Main outcome of study (acc. to author)

No significant changes in gene expression in glioblastoma and monocytic cells resulting from 1.9 GHz radiofrequency field exposures were found.

Study character:

Study funded by

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