Study type: Medical/biological study (experimental study)

Gene Expression Analysis of a Human Lymphoblastoma Cell Line Exposed In Vitro to an Intermittent 1.9 GHz Pulse-Modulated Radiofrequency Field. med./bio.

Published in: Radiat Res 2006; 165 (4): 424-429

Aim of study (acc. to author)

To study whether non-thermal radiofrequency fields of the type used for wireless communications could elicit a cellular stress response in exponentially growing TK6 cells.

Background/further details

The positive controls were maintained at 43°C for one hour.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 1.9 GHz
Modulation type: pulsed
Exposure duration: intermittent, 5 min on/10 min off, for 6 h

Exposure 1

Main characteristics
Frequency 1.9 GHz
Type
Charakteristic
  • guided field
Polarization
  • circular
Exposure duration intermittent, 5 min on/10 min off, for 6 h
Modulation
Modulation type pulsed
Exposure setup
Exposure source
Chamber The temperature in the cell cultures was maintained at 37.0 ± 0.5°C.
Setup Cells were exposed in 10 ml of culture medium in 60-mm Petri dishes using a series of circularly polarized cylindrical waveguide applicators.
Sham exposure A sham exposure was conducted.
Additional info Negative and positive (heat shock) controls were run concurrently with each experiment.
Parameters
Measurand Value Type Method Mass Remarks
SAR 1 W/kg mean unspecified - ±24%
SAR 10 W/kg mean unspecified - ±24%

Reference articles

  • Gajda GB et al. (2002): Cylindrical waveguide applicator for in vitro exposure of cell culture samples to 1.9-GHz radiofrequency fields.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • before exposure
  • after exposure

Main outcome of study (acc. to author)

Transcript levels of the investigated genes in radiofrequency exposed cells showed no significant difference in relation to the sham exposure group. However, concurrent positive (heat shock) control samples displayed a significant elevation in the expression of hsp27, hsp70, fos and jun. Conversely, the levels of myc mRNA declined in the positive control (heat shock).
In conclusion, the data revealed no evidence that the 1.9 GHz radiofrequency exposure caused a general stress response in TK6 cells under these experimental conditions.

Study character:

Study funded by

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