Study type: Medical/biological study (experimental study)

Evaluation of genotoxic effect of low level 50 Hz magnetic fields on human blood cells using different cytogenetic assays. med./bio.

Published in: Bioelectromagnetics 2004; 25 (8): 613-619

Aim of study (acc. to author)

To study whether extremely low frequency magnetic fields may contribute to mutagenesis or carcinogenesis.
In a previous study (publication 10460) the authors have found that exposure of unstimulated human blood cells to 50 Hz, 1 mT for 2 h did not induce any DNA damage. The aim of this investigation is to study the potential genotoxic effect of a longer exposure time on PHA-stimulated human blood cells.

Background/further details

In order to evaluate the possible genotoxic effects of extremely low frequency magnetic fields, human blood cells were exposed in vitro. Extremely low frequency pretreated cells were also irradiated with 1 Gy of X-ray to study the possible combined effect of extremely low frequency magnetic fields and ionizing radiation.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 48 h

General information

Each sample was divided into four aliquots namely control, positive control (12 Gy X-ray), ELF (extremly low frequency) exposed and ELF exposure followed by 1 Gy of X-ray.

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 48 h
Exposure setup
Exposure source
Chamber Incubator maintained at 37°C and 5%CO2
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - measured - 75 V/m corresponding electric field

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Data do not evidence any DNA damage induced by extremely low frequency magnetic fields exposure or any effect on cell proliferation. Results obtained from the combined exposure (extremely low frequency magnetic fields and ionizing radiation) do not suggest any synergistic or antagonistic effect.

Study character:

Study funded by

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