研究のタイプ: 医学／生物学の研究 (experimental study)

[高電力マイクロ波パルスへのインビトロばく露後のヒト白血球およびリンパ球に直接的DNA損傷なし] med./bio.

Lack of direct DNA damage in human blood leukocytes and lymphocytes after in vitro exposure to high power microwave pulses

著者: Chemeris NK, Gapeyev AB, Sirota NP, Gudkova OY, Tankanag AV, Konovalov IV, Buzoverya ME, Suvorov VG, Logunov VA
掲載誌: Bioelectromagnetics 2006; 27 (3): 197-203
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この研究は、高電力マイクロ波パルス（HPMP：8.8 GHz、パルス幅180 ナノ秒、ピーク電力65 kW、パルス繰り返し周波数50 Hz）が、ヒトの全血白血球または単離したリンパ球に遺伝毒性的影響を与えるか否かを、アルカリ単一細胞ゲル電気泳動アッセイ（アルカリコメットアッセイ）を用いて調べた。方形導波管内にて標本に40分間 のHPMPばく露を与えた。標本の温度動態から計算した平均SARは約1.6 kW/kg (ピークSARは約300 MW/kg)であった。HPMPにばく露した50μlの標本の温度上昇は、安定状態で3.5±0.1 ℃であった。その結果、HPMPばく露群では、アルカリコメットアッセイにより検出されるべきDNA損傷は､何も検出されなかった、と報告している。

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研究目的（著者による）

To study the effects of high power microwave pulses on DNA of human blood leukocytes and isolated lymphocytes.

詳細情報

At the beginning of exposure, temperature of the cell suspension was 23+/-1°C. Then the temperature increased for 10-15 s to the steady state level of 27+/-1°C, which was unchanged until the end of exposure.
Temperature and negative controls were performed at 23+/-1°C (steady state temperature of the waveguide; negative control), at 27+/-1°C (temperature increment during exposure/temperature of the exposed samples = temperature control), and at 37°C (normal physiological temperature; negative control).
Positive controls were treated with the alkylating agent ethyl methanesulfonate.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 8.8 GHz
Modulation type: pulsed
ばく露時間: continuous for 40 min
  • SAR: 1.6 kW/kg average over time

ばく露1

主たる特性
周波数 8.8 GHz
特性
  • guided field
ばく露時間 continuous for 40 min
Additional information reference article: Lebedev IV. 1961. Techniques and devices for super high frequencies, Vol. 1 Moscow-Leningrad: Gosenergoizdat.
Modulation
Modulation type pulsed
Pulse width 180 ns
Rise time 10 ns
Fall time 33 ns
Repetition frequency 50 Hz
Pulse type rectangular
ばく露装置
ばく露の発生源／構造
  • 導波管
  • rectangular, 23 x 3.4 mm, see chamber and setup details
チャンバの詳細 The cell suspension was exposed to high power microwave pulses (HPMP) in a special plastic cuvette of cylindrical shape with a diameter of 10 mm and a height of 1 mm. The cuvette was placed inside a rectangular waveguide (cross-section 23 x 3.4 mm) at a distance of 25-30 mm from a waveguide flange with the help of a special foam-plastic holder.
Additional information Calculations of the magnitude of the electric field in a rectangular waveguide operating in the H10 mode were carried out for the exterior and interior of the cuvette according to Lebedev [1961] (see reference article); the maximal magnitude of the electric field in the cuvette was about 70 kV/m.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 1.6 kW/kg average over time 計算値 - -
Additional parameter details

The average SAR of 1.6 kW/kg indicated applies to the center of the sample. Maximal SAR in the cell medium toward the microwave source was approximately by 1.7 times higher than the average SAR.

測定および計算の詳細

Temperature measurements were carried out in the cuvette filled with 50 µl of physiological saline using a copper-constantan micro-thermocouple with a diameter of 50 µm. The accuracy of temperature readings was ±0.1 °C. A thermocouple was inserted into a modified cuvette from the bottom through a narrow slot in a wide wall of the waveguide. A digital voltmeter was used for the measurements of voltage generated by a thermocouple. The time constant of the temperature measuring system was 0.05 s. The average initial rate of temperature rise was 0.37 ± 0.01 °C/s, which corresponded to the average SAR of 1.6 kW/kg (peak SAR was assessed as about 300 MW/kg).

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

The data did not reveal any statistically significant DNA damage manifested immediately after in vitro high power microwave pulses exposure of human blood leukocytes or lymphocytes or after high power microwave pulse exposure of leukocytes subsequently incubated at 37°C for 30 min compared to the respective negative (23 and 37°C) and temperature (27°C) controls.

研究の種別:

研究助成

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