研究のタイプ: 医学／生物学の研究 (experimental study)

[マイクロ波放射とインビトロでの肝臓リソゾームからの酵素放出] med./bio.

Microwave irradiation and in vitro release of enzymes from hepatic lysosomes

著者: Galvin MJ, Parks DL, McRee DI
掲載誌: Radiat Environ Biophys 1980; 18 (2): 129-136
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この研究は、2つのリソソーム酵素の放出に対する2.45GHz CWマイクロ波の影響を試験した。結果として、SAR値10、50または100mW / g（90分）では、37℃（pH7.3）でのリソソームの脆弱性（リソソーム酵素カテプシンDおよびベータ・グルクロニダーゼの放出によって決定された）への影響は見られなかった；培地をpH5.0に調整した場合、リソソーム懸濁液へのマイクロ波照射は、酸性によるリソソーム酵素放出増強効果に影響しなかった、と報告している。

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研究目的（著者による）

To investigate the effects of microwave exposure on lysosomes.

詳細情報

Lysosomes were isolated from giunea pig liver tissue. The integrity of the lysosomal membrane was determined by measuring the release of two lysosome specific enzymes, β-glucuronidase and cathepsin D.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 2.45 GHz
Modulation type: CW
ばく露時間: continuous for 90 min
neutral medium (pH = 7.3)
  • SAR: 10 mW/g mean (±3%)
  • SAR: 50 mW/g mean (±3%)
  • SAR: 100 mW/g mean (±3%)
ばく露2: 2.45 GHz
Modulation type: CW
ばく露時間: continuous for 60 min
acidic medium (pH = 5.0)
  • SAR: 10 mW/g mean (±3%)
  • SAR: 50 mW/g mean (±3%)
  • SAR: 100 mW/g mean (±3%)

ばく露1

主たる特性
周波数 2.45 GHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 90 min
Additional information neutral medium (pH = 7.3)
Modulation
Modulation type CW
ばく露装置
ばく露の発生源／構造
チャンバの詳細 The waveguide chamber was filled with distilled water (circulated and maintained at 37 °C) and matched to air by a quarter-wavelength dielectric plate (reflected power below 5%).
ばく露装置の詳細 The sample tubes of 1.06 cm inner and 1.36 cm outer diameter containing 4.2 ml of cell suspension (pH = 7.3) were located in the center of the waveguide parallel to the electric field. The exposed sample tube was placed against the dielectric plate, and the control was placed 9.5 cm away (five times the penetration depth), so it received no irradiation.
Sham exposure A sham exposure was conducted.
Additional information The maximum temperature difference between exposed and control suspension was 0.2 °C. The suspensions were stirred by small magnetic stirrers inserted in the portion of the tubes extending below the waveguide. A resulting magnetic field intensity of approximately 0.1 nT measured just above the stirrer using a circular loop was considered not to have any effect.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 10 mW/g mean 測定値 - ±3%
SAR 50 mW/g mean 測定値 - ±3%
SAR 100 mW/g mean 測定値 - ±3%
Additional parameter details

The SARs indicated are the average values measured in samples stirred at 200 rpm to reduce temperature variations [Livingston et al., 1979].

測定および計算の詳細

SARs were determined from initial-heating, time-temperature profiles [McRee, 1974] for both non-stirred and stirred suspensions with the water not circulating using high power levels and a 1-mm non-interacting probe. For the non-stirred condition, the measured SARs agreed within ±10% with the results of power loss measurements [Chou at al., 1978; Johnson et al., 1972].

ばく露2

主たる特性
周波数 2.45 GHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 60 min
Additional information acidic medium (pH = 5.0)
Modulation
Modulation type CW
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
ばく露装置の詳細 acidic medium (pH = 5.0)
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 10 mW/g mean 測定値 - ±3%
SAR 50 mW/g mean 測定値 - ±3%
SAR 100 mW/g mean 測定値 - ±3%

Reference articles

  • Livingston G et al. (1979): [チャイニーズハムスター卵巣（CHO）細胞の生存率に対する温水またはマイクロ波によるハイパサーミアの影響の比較]
  • Chou CK et al. (1978): [単離された神経および筋の標本に対する電磁界の影響]
  • McRee DI (1974): [2450 MHzマイクロ波界における生物試料によるマイクロ波放射吸収の特定]
  • Johnson CC et al. (1972): [生物学的材料およびシステムにおける非電離電磁波の影響]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

The results reveal that microwave exposure has no effect on the lysosomal membrane as indicated by the unaltered release of specific enzymes compared to unexposed control probes.

研究の種別:

研究助成