研究のタイプ: 医学/生物学の研究 (experimental study)

[テラヘルツ放射に反応した哺乳類幹細胞での再プログラミング] med./bio.

Mammalian Stem Cells Reprogramming in Response to Terahertz Radiation.

掲載誌: PLoS One 2010; 5 (12): e15806

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研究目的(acc. to editor)

To study the effects of extended exposure to a broad-spectrum Terahertz irradiation on cellular functions that are closely related to gene transcription in mouse stem cells.

詳細情報

Additionally, molecular dynamics computer simulations were performed.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 10 THz
Modulation type: pulsed
ばく露時間: continuous for 2, 4, 6 and 9 h

General information

broad-spectrum THz radiation centered at ~10 THz

ばく露1

主たる特性
周波数 10 THz
タイプ
  • electromagnetic field
ばく露時間 continuous for 2, 4, 6 and 9 h
Modulation
Modulation type pulsed
Repetition frequency 1 kHz
Additional information

pulse duration: 35 fs

ばく露装置
ばく露の発生源/構造
  • near infrared laser, frequency-doubling BBO crystal, plasma filament, Argon gas at 600 torr
ばく露装置の詳細 field directed at the bottom of the parafilm-sealed culture dish; irradiated and control dishes in thermal contact
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電力 30 MW peak - - per pulse
電力密度 1 mW/cm² average over time - - broadband

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Lipid droplet-like inclusions appeared in the cellular cytoplasm after 6 hours of exposure. Extended exposure (9 h) resulted in specific changes in cellular functions that are closely related to gene transcription. The biochip data showed that whereas 89% of the genes in mouse stem cells did not respond to the applied terahertz exposure, certain genes are activated (approx. 6% of the genes, upregulation more than two times), while other are repressed (approx. 5% of the genes, downregulation more than two times). RT-PCR experiments with selected gene probes corresponding to transcripts in the three groups of genes (i.e. of upregulated, downregulated genes and unchanged regulation) confirmed the gene specific effect. The response was not only gene specific but also exposure conditions (i.e. exposure duration) dependent.
The findings suggest that the applied terahertz irradiation accelerates cell differentiation toward adipose phenotype by activating the transcription factor peroxisome proliferator-activated receptor gamma (PPARG). Finally, the molecular dynamics computer simulations indicate that the local breathing dynamics of the PPARG promoter DNA coincides with the gene specific response to the terahertz irradiation.
The authors propose that terahertz exposure is a potential tool for cellular reprogramming.

研究の種別:

研究助成

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