この研究は、神経細胞および線維芽細胞を用いた2つの実験を行い、非熱レベルのパルス変調RF信号（PRF）が細胞の一酸化窒素（NO）産生に与える変化を調べた。PRFは、パルス幅2 ms、パルス繰り返し周波数2 Hzのパルスで変調された27.12 MHz電磁界で、磁束密度ピーク値は2.5 μT、平均電界強度は41 V/ mである。最初の実験は、ドーパミン作動性細胞株であるマウスMN9D細胞にリポ多糖（LPS）を投与する前に、RFRばく露を2分間与えた。第2に実験は、ヒト線維芽細胞培養にPRFばく露を15分間与えた。その結果、最初の実験で、RFRばく露がMN9D細胞からの一酸化窒素（NO）産生を即時に約3倍増加させることが示された；NOを、NO選択膜電極を使用してリアルタイムで電気化学的測定したところ、LPS投与後の最初の数秒以内にPRF効果が生じることが示された；第2の実験で、PRF信号がNOをほぼ2倍増加させることが示された；PRFの作用部位がカモジュリン（CaM）に関与していることを調べるために、CaMアンタゴニストW-7をPRFばく露の3時間前に培養物に加えると、PRFのNO産生増加効果がW-7によってブロックされる可能性が示された、と報告している。
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To examine the effect of a non-thermal pulsed electromagnetic field on the release of nitric oxide in neuronal cells chellenged with lipopolisaccharides (LPS) and the involvement of calcium/calmodulin as a response to such a field in fibroblasts.
Several cellular, animal and clinical studies, using electromagnetic signals have reported significant effects on tissue repair, angiogenesis, pain and inflammation. To further understand these mechanisms, two experimants were performed:
For the first experiment, neuronal cells were taken out of the incubator (37°C) and the culture medium was replaced with saline (23°C). Hence, cells were challenged through heat shock and low serum. After three minutes, a non toxic concentration of lipopolysaccharides was added to the cells. The electromagnetic field was turned on two minutes prior to the introduction of lipopolysaccharides. Nitric oxide was measured electrochemically in real-time.
For the second experiment, fibroblasts cultures, containing calf serum (increases cytosolic Ca2+ concentration) were allowed to grow in an incubator for 24 h. Three hours prior to electromagnetic field exposure, the calmodulin antagonist W-7 was added at different concentrations. After a 15 minutes exposure, nitric oxide was measured.
| ばく露 | パラメータ |
|---|---|
|
ばく露1:
27.12 MHz
Modulation type:
pulsed
ばく露時間:
2 min prior to LPS administration
MN9D cells
|
|
|
ばく露2:
27.12 MHz
Modulation type:
pulsed
ばく露時間:
continuous for 15 min
fibroblasts
|
| 周波数 | 27.12 MHz |
|---|---|
| タイプ |
|
| ばく露時間 | 2 min prior to LPS administration |
| Additional information | MN9D cells |
| Modulation type | pulsed |
|---|---|
| Pulse width | 2 ms |
| Repetition frequency | 2 Hz |
| ばく露の発生源／構造 | |
|---|---|
| ばく露装置の詳細 | single turn coil with a diameter of 20 cm; a) for studies at 37°C: coil placed inside an incubator equipped with a single plastic shelf b) for studies at 23 ± 1°C: coil placed on a non-metallic laboratory bench |
| 周波数 | 27.12 MHz |
|---|---|
| タイプ |
|
| ばく露時間 | continuous for 15 min |
| Additional information | fibroblasts |
| Modulation type | pulsed |
|---|---|
| Pulse width | 2 ms |
| Repetition frequency | 2 Hz |
| ばく露の発生源／構造 |
|
|---|
Regarding the first experiment, the release of nitric oxide after administration of an acute non-toxic concentration of lipopolysaccharide was approximately three-fold higher in exposed neuronal cells than in control cells.
In the second experiment, the release of nitric oxide was increased approximately two-fold in exposed samples compared to control samples. This effect was blocked by the calmodulin antagonist W-7.
The authors conclude that the experimental results reported in this study provide support for calcium/calmodulin-dependent nitric oxide production as an important mediator of electromagnetic field signaling that may explain the observed effects of electromagnetic fields on tissue repair, angiogenesis, pain and inflammation in animal models and clinical studies.
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