研究のタイプ: 医学/生物学の研究 (experimental study)

[電磁界はばく露された生物の一酸化窒素シグナル伝達を瞬間的に変化させる] med./bio.

Electromagnetic fields instantaneously modulate nitric oxide signaling in challenged biological systems.

掲載誌: Biochem Biophys Res Commun 2012; 426 (3): 330-333

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研究目的(著者による)

To examine the effect of a non-thermal pulsed electromagnetic field on the release of nitric oxide in neuronal cells chellenged with lipopolisaccharides (LPS) and the involvement of calcium/calmodulin as a response to such a field in fibroblasts.

詳細情報

Several cellular, animal and clinical studies, using electromagnetic signals have reported significant effects on tissue repair, angiogenesis, pain and inflammation. To further understand these mechanisms, two experimants were performed:
For the first experiment, neuronal cells were taken out of the incubator (37°C) and the culture medium was replaced with saline (23°C). Hence, cells were challenged through heat shock and low serum. After three minutes, a non toxic concentration of lipopolysaccharides was added to the cells. The electromagnetic field was turned on two minutes prior to the introduction of lipopolysaccharides. Nitric oxide was measured electrochemically in real-time.
For the second experiment, fibroblasts cultures, containing calf serum (increases cytosolic Ca2+ concentration) were allowed to grow in an incubator for 24 h. Three hours prior to electromagnetic field exposure, the calmodulin antagonist W-7 was added at different concentrations. After a 15 minutes exposure, nitric oxide was measured.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 27.12 MHz
Modulation type: pulsed
ばく露時間: 2 min prior to LPS administration
MN9D cells
  • 磁束密度: 2.5 µT peak (in the middle of a plane 1 cm from the coil's surface)
  • 電界強度: 41 V/m mean (± 10 V/m in the cells at the bottom of a 35 mm petri dish)
ばく露2: 27.12 MHz
Modulation type: pulsed
ばく露時間: continuous for 15 min
fibroblasts
  • 磁束密度: 2.5 µT peak (in the middle of a plane 1 cm from the coil's surface)
  • 電界強度: 41 V/m mean (± 10 V/m in the cells at the bottom of a 35 mm petri dish)

ばく露1

主たる特性
周波数 27.12 MHz
タイプ
  • electromagnetic field
ばく露時間 2 min prior to LPS administration
Additional information MN9D cells
Modulation
Modulation type pulsed
Pulse width 2 ms
Repetition frequency 2 Hz
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 single turn coil with a diameter of 20 cm; a) for studies at 37°C: coil placed inside an incubator equipped with a single plastic shelf b) for studies at 23 ± 1°C: coil placed on a non-metallic laboratory bench
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2.5 µT peak 測定値 - in the middle of a plane 1 cm from the coil's surface
電界強度 41 V/m mean 測定値 - ± 10 V/m in the cells at the bottom of a 35 mm petri dish

ばく露2

主たる特性
周波数 27.12 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 15 min
Additional information fibroblasts
Modulation
Modulation type pulsed
Pulse width 2 ms
Repetition frequency 2 Hz
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2.5 µT peak 測定値 - in the middle of a plane 1 cm from the coil's surface
電界強度 41 V/m mean 測定値 - ± 10 V/m in the cells at the bottom of a 35 mm petri dish

Reference articles

  • Pilla A et al. (2011): [生物学的信号伝達の最初のメッセンジャとしての電磁界:組織の修復におけるカルモジュリン依存性信号伝達への応用]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

Regarding the first experiment, the release of nitric oxide after administration of an acute non-toxic concentration of lipopolysaccharide was approximately three-fold higher in exposed neuronal cells than in control cells.
In the second experiment, the release of nitric oxide was increased approximately two-fold in exposed samples compared to control samples. This effect was blocked by the calmodulin antagonist W-7.
The authors conclude that the experimental results reported in this study provide support for calcium/calmodulin-dependent nitric oxide production as an important mediator of electromagnetic field signaling that may explain the observed effects of electromagnetic fields on tissue repair, angiogenesis, pain and inflammation in animal models and clinical studies.

研究の種別:

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