Studientyp:
Medizinische/biologische Studie
(experimentelle Studie)
Exposure of postnatal rats to a static magnetic field of 0.14 T influences functional laterality of the hippocampal high-affinity choline uptake system in adulthood; in vitro test with magnetic nanoparticles
med./bio.
[Exposition postnataler Ratten bei einem statischen Magnetfeld von 0.14 T beeinflusst die funktionale Seitigkeit des hoch-affinen Cholin-Aufnahme-Systems des Hippokampus im Erwachsenenalter; in vitro-Test mit magnetischen Nanopartikeln]
Von:
Kristofikova Z, Cermak M, Benesova O, Klaschka J, Zach P
Veröffentlicht in: Neurochem Res 2005; 30 (2): 253-262
permanent magnet with 5 cm distance between the poles
Aufbau
Six 7 or 14 day old rats from two litters were exposed at a time by making three animals share one box with a perforated cover and placing the box between the poles of the magnet.
Zusatzinfo
Twelve 7 or 14 day old rats were used in the experiments, 6 of them were used for exposure where as the remaining 6 were placed in an identical box placed behind the exposure equipment, hence serving as control. The direction of magnetic induction vector was vertical. Positive as well negative static magnetic fields was utilized for exposure which were obtained by orienting the magnetic fields either parallel or anti-parallel to the vertical component of the geomagnetic field for positive or negative fields, respectively. This was achieved by using the north pole of the permanent magnet up and south pole of the magnet down to obtain positive fields and visa versa to obtain negative fields.
Exposure and control procedures were analogous to E1 with the only exception that the 6 animals used for exposure were from one litter. Mixed synaptosomes isolated separately from the right or left hippocampi of six 7 day, four 14 day and two 12 week old male rats were resuspended in 0.32 M sucrose and exposed to a positive or negative static magnetic field at room temperature. Mixed synaptosomes isolated separately from the right or left hippocampi of six 14 day or four 12 week old animals were resuspended in 0.32 M sucrose or on a synaptosomalsuspension containing dextran-coated superparamagnetic nano particles (0.444 mg/ml). A part of sysnaptosomes with nano particles was exposed to a positive or negative static magnetic field at room temperature.
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