Study type: Medical/biological study (experimental study)

The heat shock-induced cell cycle arrest is attenuated by weak electromagnetic fields. med./bio.

Published in: Cell Prolif 2003; 36 (2): 101-111

Aim of study (acc. to author)

To study stress induced effects in human acute leukaemia cells exposed to extremely low frequency electromagnetic fields and/or heat shock.

Background/further details

Four groups were performed: 1.) control group, 2.) extremely low frequency electromagnetic field exposure alone, 3.) heat shock at 39, 41 or 43 °C alone, and 4.) co-exposure (heat shock (41 °C) plus extremely low frequency electromagnetic field exposure).
Cells were analysed 2, 4, 6, 8, and 10 days after exposure.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 30 min

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 30 min
Exposure setup
Exposure source
Setup A plastic chamber (1800 x 1400 x 60 mm³) containing cell cultures was placed in the center of the Merritt coils.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 60 µT unspecified measured - ±0.2 µT

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Compared with control group extremely low frequency electromagnetic field exposure alone neither changed the rate of cell division nor the total number of cells or the percentage of dividing cells.
Co-exposed cells showed significant smaller fraction of cell cycle arrested cells compared with group 3 (heat shock). Additionally, an increased total number of cells and higher percentage of proliferating cells was observed in co-exposed cells compared with cells exposed to heat shock alone.

Study character:

Study funded by

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