Study type: Medical/biological study (experimental study)

Extremely low frequency magnetic fields do not induce DNA damage in human lens epithelial cells in vitro. med./bio.

Published in: Anat Rec (Hoboken) 2016; 299 (5): 688-697

Aim of study (acc. to author)

The genotoxic effects of exposure of human lens epithelial cells to a 50 Hz magnetic field should be investigated.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 2 h, 6 h, 12 h, 24 h or 48 hours

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 2 h, 6 h, 12 h, 24 h or 48 hours
Exposure setup
Exposure source
Chamber two chambers with two identical coil systems placed inside a cell culture incubator
Setup temperature was constantly monitored and controlled and differences between two chambers were <0.1°C; the external computer controlling system enabled application of blind method
Sham exposure A sham exposure was conducted.
Additional info one coil in one chamber was connected in-phase to generate the magnetic field for exposure, while there was an opposite phase connection in another chamber for sham exposure
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.4 mT - - - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No significant differences in DNA single-strand breaks or double-strand breaks were detected in exposed cells compared to cells of the control group regardless of the exposure duration.
The authors conclude that there were no genotoxic effects of exposure of human lens epithelial cells to a 50 Hz magnetic field.

Study character:

Study funded by

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