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A rectangular waveguide (300 x 300 x 1000 mm) was fabricated from 1 x 1 mm brass mesh glued to an aluminium frame, and the ends were filled with 15 cm thick carbon-impregnated, RF-absorbing foam. The amplifier output was connected through a matching network into a quarter wave mobile phoneantenna placed transverse and vertical in the centre of the waveguide. The matching network was tuned using a power/SWR meter.
Four or five mice were placed inside the waveguide in a polycarbonate cage (130 x 160 x 330 mm). The same number of mice were placed outside the waveguide in an identical cage for control.
A spectrum analyser and an E-fieldprobe were used to check the radiation levels during exposure. Prior to the experiments, a calibrated single axis probe (electrically short dipole and detector diode) was used to calibrate the E-fieldprobe and to examine the spatial field variations over a grid of 10 cm and 6 cm in the longitudinal and the transverse directions, respectively. The whole-body SAR was estimated using a value of 0.045 W/kg per mW/cm² of irradiation intensity for a prolate spheroid model of a mouse in the H orientation.