Study type: Medical/biological study (experimental study)

Influence of electromagnetic fields on reproductive system of male rats. med./bio.

Published in: Int J Radiat Biol 2013; 89 (3): 147-154

Aim of study (acc. to author)

To investigate the effects of a 10 GHz electromagnetic field on male rat's reproductive system.

Background/further details

70 days old rats were sham exposed (n=6) or exposed (n=6). After the end of the experiment, blood samples were collected for determining chromosome aberration, testosterone level and performing micronucleus test. Additionally, spermatozoa were collected for determining the caspase-3 activity and morphology, as well as performing a comet assay. The experiments were repeated thrice.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 10 GHz
Exposure duration: continuous for 2 h/day for 45 days

Exposure 1

Main characteristics
Frequency 10 GHz
Type
Exposure duration continuous for 2 h/day for 45 days
Modulation
Modulation type unspecified
Exposure setup
Exposure source
Chamber rats placed in Plexiglas cages, ventilated with 1 cm diameter holes; the cages were pasted with anechoic material (top was not covered)
Setup exposure cage was placed vertically below the antenna, so that all animals were exposed homogeneously with the same power level
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
power density 0.21 mW/cm² maximum measured - -
SAR 0.014 W/kg maximum calculated whole body -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The incidence of occurence of micronuclei increased statistically significant in the exposed group compared to the sham exposed group. No abnormalities in chromosomal number and structure was found in the exposed group in comparison to the sham exposed group. In the comet assay, a significant increase in tail length and intensity was shown in the exposed samples as compared to the sham exposed group. Sperm caspase-3 activity increased significantly in the exposure group, compared to the sham exposure group. The seminiferous tubules of the exposed group showed a significantly reduced diameter compared to them of the sham exposed groups. The serum testosterone level decreased significantly in exposed animals, as compared to sham exposed animals. The mean testicular weight of the exposed group was significantly reduced in comparison to the sham exposed group.
The authors conclude that the sperms of rats could be injured by 10 GHz electromagnetic fields.

Study character:

Study funded by

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