"decreasing sinusoidally bipolar pulses of a positive half period, with pulse frequency of 50 Hz and carrier frequency 10 Hz [Panagopoulos et al., 2000]"
high voltage pulse generator
Thawed lymphocytes suspended in ice cold PBS were exposed in microcentrifuge tubes at 5 ± 0.5°C.
Using network electricity, short-duration pulses were transformed into high voltage.
Lymphocytes were also exposed to 0, 50, 100, or 150 µmol/l H2O2or to 0, 0.8, 2.5, or 4.2 Gy γ radiation from a 60Co source at a dose rate of 3.99 Gy/min after being treated or not with the pulsed electric field.