Der Einfluss zusätzlicher externer Faktoren (synergistische Wirkungen), wie Wasserstoffperoxid (0. 50, 100 oder 150 µmol/l) und Gammastrahlung (0, 0.8, 2.5, or 4.2 Gy) wurde ebenfalls bestimmt.
"decreasing sinusoidally bipolarpulses of a positive half period, with pulse frequency of 50 Hz and carrier frequency 10 Hz [Panagopoulos et al., 2000]"
Thawed lymphocytes suspended in ice cold PBS were exposed in microcentrifuge tubes at 5 ± 0.5°C.
Aufbau
Using network electricity, short-duration pulses were transformed into high voltage.
Zusatzinfo
Lymphocytes were also exposed to 0, 50, 100, or 150 µmol/l H2O2 or to 0, 0.8, 2.5, or 4.2 Gy γ radiation from a 60Co source at a dose rate of 3.99 Gy/min after being treated or not with the pulsed electric field.
Wahab MA et al.
(2007):
Elevated sister chromatid exchange frequencies in dividing human peripheral blood lymphocytes exposed to 50 Hz magnetic fields.
Friedman J et al.
(2007):
Mechanism of short-term ERK activation by electromagnetic fields at mobile phone frequencies.
Hone P et al.
(2006):
Chromatid damage in human lymphocytes is not affected by 50 Hz electromagnetic fields.
Hone P et al.
(2003):
Possible associations between ELF electromagnetic fields, DNA damage response processes and childhood leukaemia.
Robison JG et al.
(2002):
Decreased DNA repair rates and protection from heat induced apoptosis mediated by electromagnetic field exposure.
Harada S et al.
(2001):
Effects of high ELF magnetic fields on enzyme-catalyzed DNA and RNA synthesis in vitro and on a cell-free DNA mismatch repair.
Kindzelskii AL et al.
(2000):
Extremely low frequency pulsed DC electric fields promote neutrophil extension, metabolic resonance and DNA damage when phase-matched with metabolic oscillators.
Scarfi MR et al.
(1997):
50-Hz, 1-mT sinusoidal magnetic fields do not affect micronucleus frequency and cell proliferation in human lymphocytes from normal and Turner's syndrome subjects.
Cantoni O et al.
(1996):
Effect of 50 Hz sinusoidal electric and/or magnetic fields on the rate of repair of DNA single strand breaks in cultured mammalian cells exposed to three different carcinogens: methylmethane sulphonate, chromate and 254 nm U.V. radiation.
Cantoni O et al.
(1995):
The effect of 50 Hz sinusoidal electric and/or magnetic fields on the rate of repair of DNA single/double strand breaks in oxidatively injured cells.
Scarfi MR et al.
(1993):
50 Hz AC sinusoidal electric fields do not exert genotoxic effects (micronucleus formation) in human lymphocytes.
Fiorani M et al.
(1992):
Electric and/or magnetic field effects on DNA structure and function in cultured human cells.
Frazier ME et al.
(1990):
Exposure of mammalian cells to 60-Hz magnetic or electric fields: analysis of DNA repair of induced, single-strand breaks.
Reese JA et al.
(1988):
Exposure of mammalian cells to 60-Hz magnetic or electric fields: analysis for DNA single-strand breaks.
Whitson GL et al.
(1986):
Effects of extremely low frequency (ELF) electric fields on cell growth and DNA repair in human skin fibroblasts.
Cohen MM et al.
(1986):
Effect of low-level, 60-Hz electromagnetic fields on human lymphoid cells: I. Mitotic rate and chromosome breakage in human peripheral lymphocytes.
Nordenson I et al.
(1984):
Clastogenic effects in human lymphocytes of power frequency electric fields: in vivo and in vitro studies.
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