Der Einfluss zusätzlicher externer Faktoren (synergistische Wirkungen), wie Wasserstoffperoxid (0. 50, 100 oder 150 µmol/l) und Gammastrahlung (0, 0.8, 2.5, or 4.2 Gy) wurde ebenfalls bestimmt.
"decreasing sinusoidally bipolarpulses of a positive half period, with pulse frequency of 50 Hz and carrier frequency 10 Hz [Panagopoulos et al., 2000]"
Thawed lymphocytes suspended in ice cold PBS were exposed in microcentrifuge tubes at 5 ± 0.5°C.
Aufbau
Using network electricity, short-duration pulses were transformed into high voltage.
Zusatzinfo
Lymphocytes were also exposed to 0, 50, 100, or 150 µmol/l H2O2 or to 0, 0.8, 2.5, or 4.2 Gy γ radiation from a 60Co source at a dose rate of 3.99 Gy/min after being treated or not with the pulsed electric field.
Wahab MA et al.
(2007):
Elevated sister chromatid exchange frequencies in dividing human peripheral blood lymphocytes exposed to 50 Hz magnetic fields.
Friedman J et al.
(2007):
Mechanism of short-term ERK activation by electromagnetic fields at mobile phone frequencies.
Hone P et al.
(2006):
Chromatid damage in human lymphocytes is not affected by 50 Hz electromagnetic fields.
Hone P et al.
(2003):
Possible associations between ELF electromagnetic fields, DNA damage response processes and childhood leukaemia.
Robison JG et al.
(2002):
Decreased DNA repair rates and protection from heat induced apoptosis mediated by electromagnetic field exposure.
Harada S et al.
(2001):
Effects of high ELF magnetic fields on enzyme-catalyzed DNA and RNA synthesis in vitro and on a cell-free DNA mismatch repair.
Kindzelskii AL et al.
(2000):
Extremely low frequency pulsed DC electric fields promote neutrophil extension, metabolic resonance and DNA damage when phase-matched with metabolic oscillators.
Scarfi MR et al.
(1997):
50-Hz, 1-mT sinusoidal magnetic fields do not affect micronucleus frequency and cell proliferation in human lymphocytes from normal and Turner's syndrome subjects.
Cantoni O et al.
(1996):
Effect of 50 Hz sinusoidal electric and/or magnetic fields on the rate of repair of DNA single strand breaks in cultured mammalian cells exposed to three different carcinogens: methylmethane sulphonate, chromate and 254 nm U.V. radiation.
Cantoni O et al.
(1995):
The effect of 50 Hz sinusoidal electric and/or magnetic fields on the rate of repair of DNA single/double strand breaks in oxidatively injured cells.
Scarfi MR et al.
(1993):
50 Hz AC sinusoidal electric fields do not exert genotoxic effects (micronucleus formation) in human lymphocytes.
Fiorani M et al.
(1992):
Electric and/or magnetic field effects on DNA structure and function in cultured human cells.
Frazier ME et al.
(1990):
Exposure of mammalian cells to 60-Hz magnetic or electric fields: analysis of DNA repair of induced, single-strand breaks.
Reese JA et al.
(1988):
Exposure of mammalian cells to 60-Hz magnetic or electric fields: analysis for DNA single-strand breaks.
Whitson GL et al.
(1986):
Effects of extremely low frequency (ELF) electric fields on cell growth and DNA repair in human skin fibroblasts.
Cohen MM et al.
(1986):
Effect of low-level, 60-Hz electromagnetic fields on human lymphoid cells: I. Mitotic rate and chromosome breakage in human peripheral lymphocytes.
Nordenson I et al.
(1984):
Clastogenic effects in human lymphocytes of power frequency electric fields: in vivo and in vitro studies.
Um diese Webseite für Sie optimal zu gestalten und fortlaufend verbessern zu können, verwenden wir Cookies. Durch die weitere Nutzung der Webseite stimmen Sie der Verwendung von Cookies zu.