Study type: Medical/biological study (experimental study)

Effects of extremely low-frequency electromagnetic fields on delayed chromosomal instability induced by bleomycin in normal human fibroblast cells. med./bio.

Published in: J Toxicol Environ Health A 2007; 70 (15-16): 1252-1258

Aim of study (acc. to author)

To study the interaction of extremely low frequency electromagnetic fields on delayed chromosomal instability (with and without bleomycin) in human fibroblast cells.

Background/further details

Electromagnetic fields of 0.8 mT field strength was applied either alone or with bleomycin throughout the culture period. The frequencies of micronuclei and aneuploidy were analyzed at 28, 88, and 240 h after bleomycin treatment.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: continuous throughout the cultivation period

General information

cells were treated: i) with sham EMF exposure ii) with EMF exposure iii) with EMF exposure and bleomycin

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Exposure duration continuous throughout the cultivation period
Exposure setup
Exposure source
Setup two solenoids, each 0.3 m long with a diameter of 0.15 m and 350 turns/m of number 16 bifilar magnet wire
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.8 mT - - - -

Reference articles

  • Walleczek J et al. (1999): Increase in radiation-induced HPRT gene mutation frequency after nonthermal exposure to nonionizing 60 Hz electromagnetic fields.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The co-exposure (bleomycin and electromagnetic field) led to a significant increase in the frequencies of micronuclei and aneuploidy compared to the cells treated with bleomycin alone.
However, no difference was found between electromagnetic field exposed and sham exposed control cells.
The frequency of micronuclei induced by bleomycin was increased at 28 h, and persistently increased up to 240 h, but the new levels were not significantly different from the level at 28 h (= no delayed chromosomal instability by electromagnetic fields). Bleomycin alone increased the frequencies of aneuploidy at 28, 88, and 240 h, and significantly higher frequency of aneuploidy was found in the cells analyzed at 240 h compared to the cells examined at 28 h.
The data suggest that the extremely low frequency electromagnetic field enhances the cytotoxicity of bleomycin. Bleomycin might induce delayed chromosomal instability, but no effect of extremely low frequency electromagnetic field was found on the bleomycin-induced delayed chromosomal instability in fibroblast cells.

Study character:

Study funded by

Related articles