Study type: Medical/biological study (experimental study)

Effects of 60-Hz magnetic fields on DNA damage responses in HT22 mouse hippocampal cell lines. med./bio.

Published in: JEES 2015; 15 (3): 123-128

Aim of study (acc. to author)

The genotoxic effects of exposure of mouse neuronal cells to a 60 Hz magnetic field should be investigated.

Background/further details

In previous studies by the authors, the genotoxic effects of extremely low frequency magnetic fields in several epithelial and fibroblast cell lines had been investigated (Lee et al. 2012, Jin et al. 2014, Jin et al. 2012, Jin et al. 2015, Yoon et al. 2014). With this article, the study series has been expanded by research on neuronal cells.
Mouse hippocampus neuronal cells were divided into two groups: exposure to the magnetic field and sham exposure.
A positive control was performed. All results were obtained from at least three replicate experiments.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: continuous for 4, 6, 12, 16 or 24 hours

General information

positive controls were exposed to gamma radiation (10 Gy as a single dose at a rate of 5 Gy/min)

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Exposure duration continuous for 4, 6, 12, 16 or 24 hours
Exposure setup
Exposure source
Chamber exposure chamber in CO2 incubator
Setup cells in 100 mm cell culture dishes were placed in the exposure chamber inside the incubator; the exposure chamber was equipped with coils and was shielded; during exposure, the temperature in the chamber was maintained at 37 ± 0.3°C by circulating water and the temperature of the culture medium was monitored at 2-hour intervals; the incubator had a humidified atmosphere of 95% air and 5% CO2
Sham exposure A sham exposure was conducted.
Additional info sham exposed cells were treated in another incubator under the same experimental procedures with the exception of ELF-MF exposure
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2 mT - - - -

Reference articles

  • Lee HJ et al. (2012): Combined effects of 60 Hz electromagnetic field exposure with various stress factors on cellular transformation in NIH3T3 cells.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

After 6 hours of exposure, cell numbers were significantly higher in the exposure group compared to the sham exposure group. All other parameters did not show any significant differences at any time point between exposed and sham exposed cells.
The authors conclude that exposure of mouse neuronal cells to a 60 Hz magnetic field has no genotoxic effects. In synopsis with the previous studies, they see a varying sensitivity of different cell lines to genotoxic effects.

Study character:

Study funded by

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