To evaluate the inportance of gestational age of pregnant mice with regard to the exposure of 20 kHz electromagnetic field on embryogenesis.
In a previous study (publication 10485 ) with 6.26 µT (limit value for general population), the authors did not find an effect on the fetal development of pregnant mice.
In this study, the intensity was increased up to 30 µT (limit value for occupational exposure). Pregnant mice were exposed (n=10) and sham exposed (n=11) from gestation day 2.5-15.5. On gestation day 18, the dams were sacrificed.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
20 kHz
Exposure duration:
8 h/day from day 2.5 to day 15.5 of gestation
|
|
| Frequency | 20 kHz |
|---|---|
| Type | |
| Exposure duration | 8 h/day from day 2.5 to day 15.5 of gestation |
| Exposure source |
|
|---|---|
| Setup | horizontal coils consisting of 1.2 mm enamel-coated copper wire for whole body exposure |
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 30 µT | peak value | measured | - | - |
No significant effects of exposure on the developmental stage of the fetuses in pregnant mice were found. No difference was observed for the sex ratio between exposed and sham exposed mice.
Electromagnetic field exposure in pregnant mice did not affect fetal external and internal development. Thus, the data confirm the findings from a previous study (publication 10485).
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