Study type: Medical/biological study (experimental study)

Proliferation and apoptosis in a neuroblastoma cell line exposed to 900 MHz modulated radiofrequency field. med./bio.

Published in: Bioelectromagnetics 2006; 27 (3): 164-171

Aim of study (acc. to author)

To study whether a modulated radiofrequency of the type used in cellular phone communications at a specific absorption rate higher than International Commission on Non-Ionizing Radiation Protection (ICNIRP) reference level for occupational exposure, could elicit alterations on the three principal cellular processes as cell proliferation, cell differentiation, and apoptosis processes in a neuroblastoma cell line.

Background/further details

Combined exposures to radiofrequency and to the differentiative agent retinoic acid or to the apoptotic inducer camptothecin were carried out to study possible interaction between electromagnetic field and chemical agents.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 900 MHz
Modulation type: pulsed
Exposure duration: continuous for 24, 48 and 72 h

Exposure 1

Main characteristics
Frequency 900 MHz
Type
Exposure duration continuous for 24, 48 and 72 h
Additional info Reference article on thermal power pulse method: Hand JW, Lagendijk JW, Bach Andersen J, Bolomey JC. 1989. Quality Assurance Guidelines for ESHO Protocols. Int J Hyperthermia 5:421-428. (publication 14294 )
Modulation
Modulation type pulsed
Additional info

GSM signal

Exposure setup
Exposure source
Chamber Four WPCs were placed into two incubators (one for RF and one for sham exposure using blind procedure). Each WPC was shielded by a 40 x 40 x 20 cm metal grid box and four blocks of foam absorber material (15 dB). The absence of any interference between the two WPCs located in the same incubator was confirmed by measurement.
Setup The WPCs consisted of two parallel plates short-circuited at the edge at four points [Laval et al., 2002]. Four Petri dishes (3.5 cm in diameter) were placed in each WPC, one of them filled only with medium for monitoring temperature. A water spiral plate jacket was positioned on the external faces of both plates of the WPC with circulating water maintaining a temperature of 37 °C in the medium.
Sham exposure A sham exposure was conducted.
Additional info As a further control, the same number of dishes were placed in another incubator without exposure system.
Parameters
Measurand Value Type Method Mass Remarks
SAR 1 W/kg mean measured and calculated - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data suggest that 900 MHz radiofrequency exposure up to 72 h does not induce significant alterations in the three principal cell activities in a neuroblastoma cell line.

Study character:

Study funded by

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