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The study investigated the ability of millimeter waves to promote the differentiation of rat primary bone marrow stromal cells into cells with a neural phenotype, in an attempt to provide experimental basis for the future clinical application.
At cell passage 3, the cells were treated by beta-mercaptoethanol (to induce cell differentiation) alone or in combination with millimeter waves.
ばく露時間: 10 minutes, three times at an interval of 2 h
cells induced by i) beta-mercaptoethanol (BME) ii) BME + millimeter waves iii) control group
|ばく露時間||10 minutes, three times at an interval of 2 h|
|ばく露装置の詳細||culture plate placed under the radiator in a thermotank at 37 °C|
The untreated bone marrow stromal cells predominately expressed nucleostemin (a marker protein of stem cells prior to differentiation). After treatment by beta-mercaptoethanol and millimeter waves, the cells exhibited a dramatic decrease in nucleostemin expression and increase in neuron-specific enolase expression (a marker protein for neurons). The differentiation rate of the cells treated with beta-mercaptoethanol and millimeter wave exposure in combination was significantly higher than that of the cells treated with beta-mercaptoethanol alone.
The authors conclude that millimeter wave exposure enhanced the inducing effect of beta-mercaptoethanol on the differentiation of bone marrow stromal cells into cells with a neural phenotype.