Study type: Medical/biological study (experimental study)

DNA damage and apoptosis in the immature mouse cerebellum after acute exposure to a 1 mT, 60 Hz magnetic field. med./bio.

Published in: Mutat Res Genet Toxicol Environ Mutagen 2002; 513 (1-2): 121-133

Aim of study (acc. to author)

To study whether an acute 2 h exposure of a magnetic field (1 mT, 60 Hz) could elicit DNA damage, and subsequently apoptosis, in the brains of immature (10 days old) mice.

Background/further details

Mice in the positive control group were exposed to X-rays (0-3 Gy).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: continuous for 2 h

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration continuous for 2 h
Exposure setup
Exposure source
Setup 4 x 4-coil Merritt sets; coil set consisting of four 40 cm x 40 cm square coils with 84 x 36 x 36 x 84 turns of AWG12 bifiliar copper wire; animals housed in 34 cm x 20cm x 13 cm plastic cages in the middle of chamber
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT mean measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

While increased DNA damage was detected by tail ratio at 2 h after magnetic field exposure, no supporting evidence of increased DNA damage was detected by the other parameters. Additionally, no similar differences were revealed using these parameters at any of the other post-exposure times (0, 4, or 24 h).
No increase in apoptosis was found in the external granule cell layer (known to undergo apoptosis caused by a variety of genotoxic chemicals and ionizing radiation) of the cerebellum of exposed mice, when compared to sham exposed mice.
These data do not support the hypothesis that acute magnetic field exposure causes DNA damage in the cerebellums of immature mice.

Study character:

Study funded by

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